Screening beneficial bacteriostatic lactic acid bacteria in the intestine and studies of bacteriostatic substances  

作　　者：Zhijing LIU Cong XU Ran TIAN Wan WANG Jiage MA Liya GU Fei LIU Zhanmei JIANG Juncai HOU 

机构地区：[1]Key Laboratory of Dairy Science,Ministry of Education,College of Food Science,Northeast Agricultural University,Harbin 150030,China [2]College of Food Science,Northeast Agricultural University,Harbin 150030,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2021年第7期533-547,共15页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：the National Key Research and Development Program of China(No.2016YFD0400605)。

摘　　要：Lactic acid bacteria(LAB)are a representative probiotic.As the dominant flora in the human intestinal tract,LAB can regulate the balance of human intestinal flora and improve host health.The purpose of this study was to isolate and screen LAB that are well suited to the intestinal characteristics of the Chinese population,with excellent probiotics and high antibacterial activity.After 16S ribosomal RNA(rRNA)homology and phylogenetic tree analysis,potential probiotics were tested for their antibacterial activity,resistance to artificial gastrointestinal fluid and drugs,surface hydrophobicity,and safety.Three strains of LAB with acid resistance,bile salt resistance,epithelial cell adhesion,and no multidrug resistance were selected:Lactobacillus salivarius,Leuconostoc lactis,and Lactobacillus paracasei.Analysis of the antibacterial active substances in the three strains and their fermentation broths revealed that the main antibacterial substances of L.lactis were organic acids,whereas those of L.salivarius and L.paracasei were organic acids and bacteriocins with broad-spectrum antibacterial activity.These three strains of probiotic LAB with high antibacterial activity were identified as bacterial resources that could potentially be used to develop probiotic preparations for the prevention and treatment of intestinal diseases caused by intestinal pathogens.

关 键 词：Intestinal lactic acid bacteria SCREENING Probiotic Bacteriostatic substance 

分 类 号：TS201.3[轻工技术与工程—食品科学]