利用石蜡切片方法观察香菇子实体发育初期组织结构  

Microscopic Observation of EarlyFruitingBody Development of Lentinula edodes bya Modified Paraffin Section Method

作　　者：万佳宁[1] 章炉军[1] 周陈力 龚明[1] 李燕[1] 杨瑞恒[1] 鲍大鹏[1] WAN Jianing;ZHANG Lujun;ZHOU Chenli;GONG Ming;LI Yan;YANG Ruiheng;BAO Dapeng(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(S outh),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室,上海201403

出　　处：《食用菌学报》2021年第4期82-90,共9页Acta Edulis Fungi

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2016)第6-1-3号];国家自然科学基金(31800015)。

摘　　要：通过采用不同透明剂(柠檬烯、Van-Clear、正丁醇、松节油和二甲苯)制备石蜡切片,观察香菇(Lentinula edodes)子实体组织结构,确定最适透明剂,并利用优化石蜡切片方法对香菇子实体发育初期组织结构进行观察。结果表明,Van-Clear为最适透明剂,以其制备的石蜡切片中香菇子实体各部分组织结构清晰、完整、无皱缩变形。香菇菌盖皮层中的菌丝横向排列,菌丝间有大小不一的空隙,最外层菌丝排列紧密;菌肉中菌丝呈无规则致密扭结状态;菌褶中菌丝纵向规律排列,菌髓为同型结构;菌柄组织横切面呈无规则束状,存在不规律的小空隙结构。香菇原基内部菌丝呈致密无序交织状态;随着其不断膨大,下部左右两端分化出菌盖,并在菌盖下部出现栅栏状细胞整齐排列的子实层;随着菌盖的发育,菌褶结构逐渐形成。Different clearing agents were compared to improve the traditional paraffin section method and then the improved method was used to observe the tissue structure of Lentinula edodes during early fruiting body development.The results showed that the best clearing agent was Van-Clear,using which the tissue structure of each part of a fruiting body was clear,complete,and had no shrinkage or deformation.Pileus peels showed that hyphae in pileus cuticle were horizontally arranged with gaps of different sizes in between,and the outermost hyphae were tightly arranged.Context hyphae were irregularly densely kinked.Gill lamella hyphae were regularly arranged in a longitudinal manner and the hymenophoral trama was homoiomerous.The transverse section of stipe tissue was irregularly bundled with irregular small gaps in between.Primordial hyphae were dense and disorderly intertwined;and as they continuously expanded,t he the left and right ends of the lower part of the primordial hyphae differentiated into pileus with palisade cells neatly arranged at the bottom.As the development of the pileus structure continued,gill tissues gradually formed.

关 键 词：香菇 Van-Clear 透明剂 子实体发育 组织结构 

分 类 号：S646.12[农业科学—蔬菜学]