血浆环状RNA_0002867对HBV相关性肝癌诊断价值的初步研究  

Plasma circular RNA_0002867 as a diagnostic biomarker for hepatitis B virus-related hepatocellular carcinoma

作　　者：何娅娣[1,2] 吴振权 侯国良[3] He Yadi;Wu Zhenquan;Hou Guoliang(Health Management Center,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院健康管理中心,广州510630 [2]佛山市第一人民医院普通内科,佛山528000 [3]佛山市第一人民医院消化内科,佛山528000

出　　处：《新医学》2021年第8期572-575,共4页Journal of New Medicine

基　　金：国家自然科学基金(81502206)。

摘　　要：目的探讨血浆环状RNA_0002867(circ_0002867)对HBV相关性肝细胞癌(肝癌)的诊断价值。方法分析已发表的测序结果,筛选在肝癌患者的组织和血浆中同时高表达的环状RNA,通过微滴数字PCR(ddPCR)检测35例HBV相关性肝癌患者(肝癌组)及30例HBV相关性肝硬化患者(肝硬化组)血浆circ_0002867表达水平,电化学发光法检测2组患者的血清甲胎蛋白水平,应用受试者工作特征(ROC)曲线分析血浆circ_0002867、血清甲胎蛋白及两者联合对HBV相关性肝癌的诊断价值。结果在肝癌患者的组织和血浆中同时高表达的环状RNA中取交集筛选出circ_0002867行进一步验证。肝癌组血浆circ_0002867表达水平为(29.63±11.56)copies/ml,高于肝硬化组的(19.15±11.57)copies/ml(P<0.001);血浆circ_0002867、血清甲胎蛋白及两者联合诊断HBV相关性肝癌的ROC曲线下面积分别为0.725(95%CI 0.600~0.828)、0.670(95%CI 0.542~0.781)、0.810(95%CI 0.693~0.896),血浆circ_0002867和血清甲胎蛋白两者联合提高了血清甲胎蛋白诊断HBV相关性肝癌的效能(P<0.05)。结论血浆circ_0002867联合血清甲胎蛋白对诊断HBV相关性肝癌具有较好的临床价值。Objective To investigate the diagnostic value of plasma circular RNA_0002867(circ_0002867)for hepatitis B virus(HBV)-related hepatocellular carcinoma(HCC).Methods The published sequencing results were analyzed to screen the high expression of circular RNAs in both tissues and plasma of HCC patients.The expression levels of plasma circ_0002867 were detected by digital droplet polymerase chain reaction(ddPCR)in 35 patients with HBV-related HCC and 30 patients with HBV-related liver cirrhosis.The serum alpha-fetoprotein(AFP)levels were determined by electrochemiluminescence in two groups.The receiver operating characteristic(ROC)curve was delineated to analyze diagnostic values of plasma circ_0002867,serum AFP and these two combined for HCC.Results The circ_0002867,which was highly expressed in both tissues and plasma of HCC patients,was screened for subsequent validation.The expression level of plasma circ_0002867 in the HBV-related liver cirrhosis group was significantly lower than that in the HBV-related HCC group((19.15±11.57)copies/ml vs.(29.63±11.56)copies/ml;P<0.001)).The area under ROC for plasma circ_0002867,serum AFP,and these two combined was 0.725(95%CI 0.600-0.828),0.670(95%CI 0.542-0.781)and 0.810(95%CI 0.693-0.896),respectively.The combination of serum AFP and plasma circ_0002867 significantly improved the diagnostic accuracy of serum AFP for HBV-related HCC(P<0.05).Conclusion Plasma circ_0002867 combined with serum AFP is a potential diagnostic biomarker for HBV-related HCC in clinical practice.

关 键 词：乙型肝炎病毒 肝细胞癌 诊断 血浆 甲胎蛋白 环状核糖核酸 

分 类 号：R735.7[医药卫生—肿瘤]