白藜芦醇调节TLR4/NF-κB信号通路影响人前列腺癌PC3细胞株凋亡的研究  

Effects of resveratrol on apoptosis of prostatic cancer PC3 cell lines via regulating human TLR4/NF-κB signaling pathway

作　　者：冉亨勇 蒲军[1] 李明辉[1] 何毅 RAN Hengyong;PU Jun;LI Minghui;HE Yi(Department of Oncology,Qionglai Municipal Medical Center Hospital,Qionglai,Sichuan 610000,China;Department of Urological Surgery,Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,China)

机构地区：[1]邛崃市医疗中心医院肿瘤科,四川邛崃610000 [2]西南医科大学附属医院泌尿外科,四川泸州646000

出　　处：《重庆医学》2021年第15期2534-2538,共5页Chongqing medicine

基　　金：泸州市-西南医科大学科技战略合作项目(2017LZXNYD-J32)。

摘　　要：目的探讨白藜芦醇(Res)影响人前列腺癌PC3细胞株增殖和凋亡的机制。方法将人前列腺癌PC3细胞株分为阴性对照组,Res高、中、低剂量组(40、20、10μmol/L Res),Toll样受体4(TLR4)阻断剂组,采用CCK-8法检测PC3细胞株细胞增殖情况;Hochest33342荧光染色检测PC3细胞株细胞凋亡情况;Western blot检测TLR4、核因子-κB(NF-κB)、Bcl-2与Bax蛋白表达;ELISA检测白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)表达。结果Res能明显抑制PC3细胞增殖;Hochest33342荧光染色结果显示Res各剂量组肿瘤细胞凋亡增加,Bcl-2/Bax比值降低,TLR4、NF-κB表达水平降低,与阴性对照组比较,Res 20、40μmol/L组差异均有统计学意义(P<0.05)。结论Res能抑制PC3细胞增殖,促进其凋亡,可能与抑制TLR4信号通路,下调Bcl-2/Bax比值,减少炎性因子表达有关。Objective To investigate the effects of resveratrol(Res)on the proliferation and apoptosis of prostatic cancer PC3 cell lines via regulating TLR4/NF-κB signaling pathway.Methods The PC3 cell lines were divided into the negative control group,Res high,middle and low dose groups(40,20,10μmol/L Res)and Toll-like receptor 4(TLR4)blocker group.The cell proliferation of PC3 cell lines was detected by the CCK-8 method.The apoptosis of PC3 cell lines was detected by the Hochest33342 fluorescence staining.The expressions of TLR4,NF-κB,Bcl-2 and Bax were detected by Western blot;the expressions of IL-6 and TNF-αwere detected by ELISA.Results Res could significantly inhibit the proliferation of PC3 cells,the Hochest33342 fluorescence staining results showed that the apoptosis of tumor cells in each Res group was increased,the ratio of Bcl-2/Bax was decreased,the expression levels of TLR4 and NF-κB were decreased,compared with the negative control group,the differences in the Res 20,40μmol/L groups had statistical sifgnificance(P<0.05).Conclusion Res can inhibit the proliferation of PC3 cells and promote their apoptosis,which may be related to inhibiting the TLR4 signal pathway,down-regulating the Bcl-2/Bax ratio and reducing the expression of inflammatory factors.

关 键 词：白藜芦醇 前列腺癌 凋亡 TOLL样受体4 

分 类 号：R737.1[医药卫生—肿瘤]