FGF2、CYSLTR1、RSAD2和HLA-DRA的表达水平与弥漫性大B细胞淋巴瘤预后的相关性  

Correlation between the expression levels of FGF2,CYSLTR1,RSAD2 and HLA-DRA and prognosis of diffuse large B-cell lymphoma

作　　者：刘利 李珊珊[1] 余楚壬 袁玉 代怀杰 曹培杰 LIU Li;LI Shan-shan;YU Chu-ren;YUAN Yu;DAI Huai-jie;CAO Pei-jie(Department of Oncology and Hematology,Dongguan Houjie Hospital,Dongguan 523945,Guangdong,CHINA)

机构地区：[1]东莞市厚街医院肿瘤血液科,广东东莞523945

出　　处：《海南医学》2021年第15期1909-1914,共6页Hainan Medical Journal

摘　　要：目的探讨免疫相关基因FGF2、CYSLTR1、RSAD2和HLA-DRA的表达水平与弥漫性大B细胞淋巴瘤(DLBCL)患者预后的相关性。方法分别从基因表达综合(GEO)和癌症基因组图集(TCGA)数据库中获得221例和48例DLBCL患者的核糖核酸(RNA)测序数据。应用单因素和多因素COX回归模型以及Kaplan-Meier曲线探索免疫相关基因的表达水平与DLBCL患者总体生存(OS)的相关性。同时,应用时间依赖性接收器工作特性曲线(ROC)评估危险分层的性能。结果单因素COX回归模型分析提示,总共有4个免疫相关基因FGF2,CYSLTR1,RSAD2和HLA-DRA的高表达与DLBCL患者的不良OS显著相关[风险比(HR)>1,P<0.05],此结果在Kaplan-Meier曲线中得到验证(P<0.05);多因素COX回归模型被进一步用于对这4个免疫基因进行联合分析,发现FGF2对OS的贡献度最大(系数β=0.27),并且可以对DLBCL患者进行危险分层;此外,单因素和多因素COX回归分析发现,危险分层是DLBCL患者独立的预后影响因子(P<0.05);同时,进一步的时间依赖性ROC曲线表明危险分层具有较好的性能[曲线下面积(AUC)>0.8]。结论FGF2、CYSLTR1、RSAD2和HLA-DRA的高表达与DLBCL患者的不良预后显著相关,同时,联合这四个免疫相关基因可以对DLBCL患者进行危险分层。Objective To explore the correlation between the expression levels of immune related genes FGF2,CYSLTR1,RSAD2 and HLA-DRA and the prognosis of patients with diffuse large B-cell lymphoma(DLBCL).Methods The ribonucleic acid(RNA)sequencing data of 221 and 48 DLBCL patients were obtained from the Gene Expression Omnibus(GEO)and the Cancer Genome Atlas(TCGA)databases,respectively.Univariate and multivariate COX regression models and Kaplan-Meier curves were used to explore the correlation between the expression levels of immune-related genes and the overall survival(OS)of DLBCL patients.Moreover,the time-dependent receiver operat-ing characteristic curve(ROC)curve was used to evaluate the performance of risk stratification.Results The result of the univariate COX regression model indicated that the high expression of four immune-related genes FGF2,CYSLTR1,RSAD2 and HLA-DRA was significantly associated with poor OS of DLBCL patients[hazard ratio(HR)>1,P<0.05].The result was confirmed by Kaplan-Meier curves(P<0.05).Meanwhile,a multivariate COX regression model was fur-ther used to analyze the combination of FGF2,CYSLTR1,RSAD2 and HLA-DRA,which found that they can perform risk stratification of DLBCL patients,and that FGF2 contributed the greatest to the OS(coefficientβ=0.27).Notably,uni-variate and multivariate COX regression analysis found that risk stratification was an independent prognostic factor for DLBCL patients.Furthermore,the time-dependent ROC curve showed that risk stratification had good performance[ar-ea under curve(AUC)>0.8].Conclusion High expression of FGF2,CYSLTR1,RSAD2 and HLA-DRA was signifi-cantly associated with poor prognosis of DLBCL patients.Furthermore,the combination of these four immune-related genes could perform risk stratification for DLBCL patients.

关 键 词：弥漫性大B细胞淋巴瘤 免疫相关基因 总体生存 危险分层 分子标志物 

分 类 号：R733.4[医药卫生—肿瘤]