检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:翟玉喜[1] 冯娜 高建鲁[1] 汪鑫[1] Zhai Yuxi;Feng Na;Gao Jianlu;Wang Xin(Department of Ophthalmology,Liaocheng People's Hospital,Liaocheng 252000,China;Department of Ophthalmology,Shenxian Central Hospital,Shenxian 252400,China)
机构地区:[1]聊城市人民医院眼科,山东聊城252000 [2]莘县中心医院眼科,山东聊城252400
出 处:《山东第一医科大学(山东省医学科学院)学报》2021年第7期516-520,共5页Journal of Shandong First Medical University & Shandong Academy of Medical Sciences
摘 要:目的测定家族性原发性开角型青光眼(primary open angle glaucoma,POAG)及散发性POAG患者泪液、血清脑源性神经营养因子(brain derived neurotrophic factor,BDNF)含量,为寻找POAG早期诊断标记物提供实验基础,并评价其与POAG分期的相关性。方法﹐收集符合纳入、排除标准的散发性POAG患者14例及山东阳谷-POAG遗传家系的6例患者(Ⅲ-8、Ⅲ-10、Ⅲ-12、Ⅲ-18、Ⅲ-20、Ⅲ-31)的临床资料、血液及泪液标本,另外与实验组年龄、性别对应的健康对照者50例,应用人单克隆抗BDNF抗体ELISA试剂盒,按照试剂盒操作步骤对各标本进行BDNF测定。统计学处理应用SPSS17.0统计软件,组间比较采用t检验,不同病程POAG患者BDNF'水平比较采用单因素方差分析,两两比较采用LSD-t检验;BDNF水平与年龄相关性分析采用Pearson相关性分析。结果﹒显性遗传家系POAG组泪液及血清BDNF较散发POAG组含量减少,但差异无统计学意义(P>0.05);POAC患者组泪液BDNF较健康对照组含量减少,差异有统计学意义(P<0.05);POAG患者组血清BDNF较健康对照组含量减少,但差异无统计学意义(P>0.05)。结论泪液,血清BDNF在该阳谷家族性POAG致病中的作用有限;血清BDNF受全身多种因素的影响,很难为POAG早期诊断提供有效信息;POAG患者基础泪液的BDNF含量明显减少,患者基础泪液BDNF有望成为POAG早期诊断标记物。Objective:To determine the BDNF levels in serum and tears of patients from pedigree and sporadic cases with POAG,which may provide experimental basis for exploring the pathogenesis of POAG and to evaluate its correlation with POAG staging.Methods:Clinical data,blood and tear samples were collected from 6 patients of one POAG pedigree(Ⅲ-8、Ⅲ-10、Ⅲ-12、Ⅲ-18、Ⅲ-20 and HI-31)and 14 sporadic cases.50 age-and sex-matched healthy controls were recruited at the same time.The levels of BDNF were tested by Elisa kit according to its instructions.Statistical analysis was performed using SPSS 17.0 statistical software,t test was used for comparison between groups,one-way analysis of variance was used for comparison of BDNF levels in POAG patients with different course of disease,and LSD-t test was used for pair comparison.The correlation between BDNF level and age was analyzed by Pearson correlation analysis.Results:The serum and tear BDNF levels in POAG pedigree patients were lower than those in sporadic POAG patients.However,the difference was not significant(P>0.05);The levels of BDNF in tears of POAG patients were significantly lower than those of normal control group,and the difference was statistically significant(P<0.05).Serum BDNF of POAG patients was lower than that of normal control group,and the difference was not significant(P>0.05).Conclusion:The role of serum and tear BDNF in the pathogenesis of POAG pedigree which has pathogenic gene mutations is limited.Serum BDNF is affected by many systemic factors,and it is difficult to provide effective information for the early diagnosis of POAG.The tear BDNF levels in patients with POAG are significantly reduced.The determination of BDNF in basic tears of patients is expected to be a marker for early diagnosis of POAG.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.249