幽门螺杆菌感染与结直肠癌EMT的相关性研究  

Relationship between Helicobacter pylori infection and epithelial-mesenchymal transition in colorectal cancer

作　　者：楼何飞 马春梅 傅英芝 张术 He-Fei Lou;Chun-Mei Ma;Ying-Zhi Fu;Shu Zhang(Clinical Laboratory of Pan’an Traditional Chinese Medical Hospital,Jinhua 322300,Zhejiang Province,China;The Affiliated Hospital of Hangzhou Normal University,Hangzhou 310015,Zhejiang Province,China)

机构地区：[1]磐安县中医院检验科,浙江省金华市322300 [2]杭州市第二人民医院,浙江省杭州市310015

出　　处：《世界华人消化杂志》2021年第15期858-865,共8页World Chinese Journal of Digestology

摘　　要：背景幽门螺杆菌(Helicobacter pylori,H.pylori)感染公认的Ⅰ类致癌因素,但其与结直肠癌(colorectal cancer,CRC)上皮-间质转化(epithelial-mesenchymal transition,EMT)的关系报道较少.故本研究推测H.pylori感染与EMT存在一定相关性,在CRC发生、发展中可能起着重要作用.目的探讨H.pylori感染与CRC患者EMT的相关性.方法选取2016-01/2017-10我院经手术组织病理检查证实为CRC患者97例,另选取同期因其他原因接受肠切除术患者118例作为对照组.比较两组基线资料、H.pylori感染、EMT相关蛋白[E-钙黏蛋白(E-cadherin,E-cad)、波形蛋白(vimentin,VIM)、β-连环素(β-catenin,β-CAT)]阳性率,探讨不同H.pylori感染状态下EMT相关蛋白表达及临床病理参数,分析H.pylori感染与EMT相关蛋白表达相关性,评价H.pylori感染与生存的关系.结果H.pylori感染、VIM、β-CAT蛋白阳性率:观察组>对照组,E-cad蛋白阳性率:观察组<对照组(P<0.05);观察组H.pylori阳性者分化程度低分化、临床分期Ⅲ+Ⅳ期、浸润深度全层/浆膜外比例、区域淋巴结转移比例、VIM、β-CAT蛋白阳性率高于H.pylori阴性者,E-cad蛋白阳性率低于H.pylori阴性者(P<0.05);H.pylori感染与E-cad蛋白呈负相关,与VIM、β-CAT蛋白呈正相关(P<0.05);H.pylori阳性表达患者3年生存率低于阴性表达患者(P<0.05).结论H.pylori感染与CRC患者EMT存在一定相关性,推测H.pylori感染可能通过促进EMT发生导致CRC侵袭、转移及不良预后.BACKGROUND Helicobacter pylori(H.pylori)has been recognized as a class I carcinogen,but its relationship with epithelial-mesenchymal transition(EMT)in colorectal cancer(CRC)T)is rarely reported.We hypothesized that H.pylori infection is related to EMT and may play an important role in the occurrence and development of CRC.AIM To investigate the relationship between H.pylori infection and EMT in CRC patients.METHODS From January 2016 to October 2017,97 patients with CRC confirmed by surgical histopathological examination at our hospital were selected,and 118 patients who underwent bowel resection for other reasons during the same period were selected as a control group.Baseline data,H.pylori infection,and the positive rates of EMT-related proteins[E-cadherin(E-cad),vimentin(VIM),andβ-catenin(β-CAT)]were compared between the two groups.The expression of EMT-related proteins and clinicopathological parameters in different H.pylori infection states were analyzed to assess the association of H.pylori infection with EMT-related protein expressionn and survival.RESULTS The positive rates of H.pylori infection and VIM andβ-CAT protein expression were significantly higher in the observation group than in the control group,while the positive rate of E-cad protein expression was significantly lower in the observation group than in the control group(P<0.05).In the observation group,significantly more H.pylori positive patients had poor differentiation,clinical stageⅢ/Ⅳ,deep infiltration(full thickness/extraserous ratio),regional lymph node metastasis,and positive VIM andβ-CAT protein erexpression than H.pylori negative ones,while E-cad protein positive rate was significantly lower than that of H.pylori negative patients(P<0.05).H.pylori infection was negatively correlated with E-cad protein expression,and positively correlated with VIM andβ-CAT protein expression(P<0.05).The 3-year survival rate of H.pylori positive patients was lower than that of H.pylori negative patients(P<0.05).CONCLUSION There is a correlation

关 键 词：幽门螺杆菌感染 结直肠癌 上皮-间质转化 E-钙黏蛋白 波形蛋白 Β-连环素 

分 类 号：R735.34[医药卫生—肿瘤]