水飞蓟素对重症急性胰腺炎大鼠肠道损伤及PI3K/Akt和NF-κB信号通路影响  

Effect of silymarin on intestinal injury and PI3K/Akt and NF-κB signaling pathways in rats with severe acute pancreatitis

作　　者：哈虹[1] 哈良 张庆清 Hong Ha;Liang Ha;Qing-Qing Zhang(Department of Gynecology,First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300191,China;First Department of Hepatobiliary and Pancreatic Medicine,Tianjin Hospital of Integrated Traditional Chinese and Western Medicine,Tianjin 300000,China;Department of Gynecology of Traditional Chinese Medicine,Community Health Service Center,Tianjin 300181,China)

机构地区：[1]天津中医药大学第一附属医院妇科,天津市300191 [2]天津市中西医结合医院肝胆胰一科,天津市300000 [3]天津市河东区中山门街社区卫生服务中心中医妇科,天津市300181

出　　处：《世界华人消化杂志》2021年第14期765-774,共10页World Chinese Journal of Digestology

摘　　要：背景急性胰腺炎是临床十分常见的急腹症,过量的炎症因子释放会损伤肠黏膜,诱导细胞凋亡,加快病程.水飞蓟素有抗氧化、免疫调节、消炎、保护肝脏、抗组织纤维化等功效.但目前尚未见关于水飞蓟素在重症急性胰腺炎肠道损伤中的相关研究.目的研究水飞蓟素对重症急性胰腺炎大鼠肠道损伤及PI3K/Akt和NF-κB信号通路的影响.方法实验分成Control组、重症急性胰腺炎(severe acute pancreatitis model,SAP)组、Experimental+L组(重症急性胰腺炎模型,给予60 mg/kg水飞蓟素治疗)、Experimental+M组(重症急性胰腺炎模型,给予120 mg/kg水飞蓟素治疗)、Experimental+H组(重症急性胰腺炎模型,给予240 mg/kg水飞蓟素治疗)、Glutamine组(1.5 g/kg谷氨酰胺治疗),观察大鼠死亡情况,全自动生化分析仪检测血清中淀粉酶、脂肪酶水平,用ELISA法检测血清中白介素(interleukin,IL)-1β、肿瘤坏死因子(tumor necrosis factor,TNF)-α含量,对大鼠进行胰腺病理评分和回肠病理评分,Western blot检测回肠组织中Bcl-2、Bax、PI3K、p-PI3K、Akt、p-Akt、NF-κBp65蛋白表达,TUNEL法检测回肠组织中细胞凋亡情况.结果SAP组大鼠死亡3只,Control、Experimental+L、Experimental+M、Experimental+H组大鼠分别死亡0只、2只、1只和0只.与Control组相比,SAP组大鼠血清中淀粉酶、脂肪酶以及IL-1β、TNF-α水平升高,胰腺病理评分和回肠病理评分均升高,Bax、p-PI3K/PI3K、p-Akt/Akt、NF-κBp65蛋白水平和细胞凋亡指数均升高,Bcl-2蛋白表达水平降低.与SAP组比较,Experimental+L、Experimental+M、Experimental+H组大鼠血清中淀粉酶、脂肪酶以及IL-1β、TNF-α水平均逐渐降低,胰腺病理评分和回肠病理评分均逐渐降低,Bax、p-PI3K/PI3K、p-Akt/Akt、NF-κBp65蛋白水平和细胞凋亡指数均逐渐降低,Bcl-2蛋白表达水平逐渐升高.与SAP组比较,Glutamine组大鼠血清中淀粉酶、脂肪酶以及IL-1β、TNF-α水平均降低,胰腺病�BACKGROUND Acute pancreatitis is a very common clinical acute abdomen.Excessive release of inflammatory factors can damage the intestinal mucosa,induce cell apoptosis,and accelerate the course of the disease.Silymarin has anti-oxidative,immunoregulatory,anti-inflammatory,liver-protecting,anti-tissue fibrosis,and other effects.However,there is no relevant research on the effect of silymarin on the intestinal injury in severe acute pancreatitis.AIM To study the effects of silymarin on intestinal injury and PI3K/Akt and NF-κB signaling pathways in rats with severe acute pancreatitis.METHODS Rats were divided into a control group,a severe acute pancreatitis(SAP)model group,and low-,medium,and high-dose silymarin groups(severe acute pancreatitis rats given 60,120,and 240 mg/kg silymarin,respectively).Rats treated with glutamine(1.5 g/kg)were used as positive controls.The death of rats was recorded.Serum amylase and lipase levels were detected using an automatic biochemical analyzer.Serum IL-1βand TNF-αlevels were detected by ELISA.Pancreatic and ileal pathology scores were scalculated.The expression of Bcl-2,Bax,PI3K,p-PI3K,Akt,p-Akt,NF-κBp65,and Bcl-2 in ileal tissues was detected by Western blot.TUNEL method was used to detect cell apoptosis in ileal tissues.RESULTS The SAP group,control group,and low-,medium,and high-dose silymarin groups had 3,0,2,1,and 0 deaths,respectively.Compared with the control group,the levels of serum amylase,lipase,IL-1β,and TNF-α,the pancreatic and ileal pathology scores,the levels of Bax,p-PI3K/PI3K,p-Akt/Akt,and NF-κBp65,and the apoptosis index increased,while Bcl-2 protein expression decreased in the SAP group.Compared with the SAP group,the levels of serum amylase,lipase,IL-1β,and TNF-αased,the pancreatic and ileal pathology scores,the levels of Bax,p-PI3K/PI3K,p-Akt/Akt,and NF-κBp65 protein levels,and the apoptosis index gradually decreased,while Bcl-2 protein expression gradually increased in the low-,medium,and high-dose silymarin groups.Compared with the SAP group,the s

关 键 词：水飞蓟素 PI3K/AKT 重症急性胰腺炎 NF-ΚB 炎症 

分 类 号：R576[医药卫生—消化系统]