Diverse microbiota in palatal radicular groove analyzed by Illumina sequencing:Four case reports  

作　　者：Xue-Lian Tan Xuan Chen Yu-Jie Fu Ling Ye Lan Zhang Ding-Ming Huang 

机构地区：[1]State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Operative Dentistry and Endodontics West China Hospital of Stomatology,Sichuan University,Chengdu 610041,Sichuan Province,China

出　　处：《World Journal of Clinical Cases》2021年第23期6846-6857,共12页世界临床病例杂志

基　　金：Supported by National Natural Science Foundation of China,No.82001037;the Research and Develop Program,West China Hospital of Stomatology,Sichuan University,No.RD-02-202007.

摘　　要：BACKGROUND A palatal radicular groove is an unusual developmental deformity of the tooth,which may serve as a channel linking the periodontal and periapical inflammation,and yet no literature could be obtained analyzing microbiota within the palatal radicular grooves.CASE SUMMARY Four patients diagnosed with palatal radicular groove and concomitant periodontal-endodontic deformity in permanent maxillary lateral incisors were enrolled in this work.Twelve bacterial samples from 4 patients were collected from different parts of the palatal radicular groove during intentional replantation surgery.Illumina sequencing was performed to analyze the taxonomical composition and microbiome structure inside the palatal grooves,and 1162 operational taxonomic units were obtained.The phyla of Firmicutes and Proteobacteria predominated in most of the samples.An unknown genus from the Bacillaceae family,Lactococcus,and Porphyromonas were the most abundant genera identified.There was no difference in the microbiota richness and diversity in three sections of the groove.CONCLUSION The unique ecological niches inside the palatal grooves harbored bacterial communities that shared some component features of both the endodontic and periodontal infections.The existence of palatal groove may play an interaction bridge between the root apex and tooth cervix and thus impair the outcome of traditional therapeutic methods such as root canal treatment and periodontal management.

关 键 词：Palatal radicular groove High-throughput sequencing Microbiota composition TAXONOMY Endodontic-periodontal infection Case report 

分 类 号：R781.2[医药卫生—口腔医学]