潮汐-复合流人工湿地系统优化及对抗生素抗性基因的去除效果  

Optimization of Tidal-Combined Flow Constructed Wetland System and Its Removal Effect on Antibiotic Resistance Genes

作　　者：程羽霄 吴丹[1,2] 陈铨乐 高方舟 杨永强[3] 刘有胜 应光国 CHENG Yu-xiao;WU Dan;CHEN Quan-le;GAO Fang-zhou;YANG Yong-qiang;LIU You-sheng;YING Guang-guo(School of Environment,South China Normal University,Guangzhou 510006,China;Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety,Key Laboratory of Theoretical Chemistry of Environment,South China Normal University,Guangzhou 510006,China;State Key Laboratory of Organic Geochemistry,Guangzhou Institute of Geochemistry,Chinese Academy of Sciences,Guangzhou 510640,China)

机构地区：[1]华南师范大学环境学院,广州510006 [2]华南师范大学广东省化学品污染与环境安全重点实验室,环境理论化学教育部重点实验室,广州510006 [3]中国科学院广州地球化学研究所,有机地球化学国家重点实验室,广州516000

出　　处：《环境科学》2021年第8期3799-3807,共9页Environmental Science

基　　金：国家自然科学基金项目(41877359);广东省自然科学基金项目(2020A1515010448)。

摘　　要：抗生素抗性基因随废水排放传播扩散,对环境生物和民众健康构成严重威胁.针对废水中抗性基因的深度去除值得重点关注.在前期研究中发现潮汐流人工湿地能有效去除废水中多种氮素.本研究通过增加隔板和种植植物等方式进一步优化潮汐流人工湿地系统,并考察了工艺优化对抗性基因去除和脱氮功能微生物的影响机制.结果表明,同时增加隔板和种植植物后的潮汐-复合流人工湿地系统能有效提高抗性基因的去除效率,在增加隔板和种植植物组对7类21种抗性基因去除率最高达到83.82%~100.0%,显著高于单一增加隔板或种植植物组.从湿地基质和植物中的抗性基因绝对丰度对比可以看出,增加隔板能促进湿地基质中抗性基因量积累,而植物对抗性基因吸附也是其去除途径之一.同时,结合氮循环功能基因测序结果显示,湿地系统优化能提高基质中硝化和反硝化功能微生物物种多样性和丰富度,这与优化系统对废水中硝化量、反硝化量和总氮的去除率相对更高结果一致.Antibiotic resistance genes(ARGs) pose a serious threat to environmental biology and public health,along with the discharge and spread of wastewater.The advanced treatment of ARGs in wastewater therefore deserves special attention.In our previous study,we found that tidal flow constructed wetlands can effectively remove multiple ammonia from wastewater.In this study,we further optimized tidal flow constructed wetland systems by adding baffles and cultivating plants;we investigated the influence of process optimization on the removal of ARGs and the influence of functional microorganism distribution on nitrogen removal.The results show that the addition of baffles and plants can effectively improve the removal efficiency of ARGs,with the maximum removal rate of 21 resistance genes,in 7 categories,reaching 83.82%-100.0% with the simultaneous addition of baffles and plants.These removal rates were significantly higher than the increase resulting from a single baffle or plant group.From the comparison of the absolute abundance of ARGs in the substrate and plants,it is clear that the baffles can promote the enrichment of ARGs in the wetland substrate,while uptake by plants is also a way of removing ARGs.Combined with the results of nitrogen-cycle functional gene sequencing,system optimization can increase the diversity and richness of nitrification and denitrification functional microorganisms in the substrate,which is consistent with the higher removal rate of nitrification and total nitrogen in wastewater.

关 键 词：人工湿地 抗生素抗性基因 去除效率 微生物多样性 硝化和反硝化 

分 类 号：X52[环境科学与工程—环境工程]