宠物猫细小病毒XNF-1株分离鉴定及VP2基因遗传变异分析  

Isolation and Identification of Canine Parvovirus XNF-1 Strain and Genetic Variation Analysis of VP2 Gene

作　　者：蒋含伟 解秀梅[1] 祁天荣 李雪梅 JIANG Han-wei;XIE Xiu-mei;QI Tian-rong;LI Xue-mei(Qinghai Vocational and Technical College of Animal Husbandry and Veterinary Medicine,Huang yuan,Qinghai.812100,China;Bai Hui Yuan Pet Hospital,Xining,Qinghai,810001,China;Qinghai Academy of Animal Husbandry and Veterinary Sciences,Xining,Qinghai,810016)

机构地区：[1]青海畜牧兽医职业技术学院,青海淳源812100 [2]佰汇缘宠物医院,青海西宁810001 [3]青海省畜牧兽医科学院,青海西宁810016

出　　处：《动物医学进展》2021年第8期64-68,共5页Progress In Veterinary Medicine

基　　金：青海省农牧厅科技项目(NMSY-2017-11)。

摘　　要：为了分析宠物猫细小病毒(FPV)流行株的VP2基因的遗传变异情况,通过病毒分离、形态学观察、PCR方法及间接免疫荧光试验(IFA)从疑似FPV感染的宠物猫粪便和血清等病料中分离得到1株FPV,命名为XNF-1株。采用人工感染试验检测其XNF-1株的致病性,采用PCR方法扩增XNF-1株VP2基因并测序,分析其遗传变异情况。结果显示,XNF-1株对猫具有致病性,XNF-1株与16株参考株VP2基因同源性在98.1%〜99.9%之间,与FPV-C株、FPV-D株、FPV-A株位于同一分支,其中与FPV-A株位于同一分支.遗传距离最近,与其他参考株遗传距离较远。研究结果为宠物猫细小病毒病防控及疫苗研究奠定了基础。In order to analyze the genetic variation of VP2 gene of pet cat parvovirus(FPV)epidemic strains,one strain of FPV,named XNF-1 strain,was isolated from feces and serum of suspected pet cat FPV by virus isolation,morphological observation,PCR and IFA.The results showed that XNF-1 strain had pathogenicity to cats.The VP2 gene homology between XNF-1 and 16 reference strains ranged from 98.1%to 99.9%.XNF-1 is located in the same branch with FPV-C,FPV-D and FPV-A strains.Among them,XNF-1 is located in the same branch with FPV-A strain and has the closest genetic distance with other reference strains.This study laid a foundation for the prevention and control of pet cat parvovirus disease and vaccine research.

关 键 词：宠物猫 猫细小病毒病 分离鉴定 VP2基因 遗传变异 

分 类 号：S852.659.2[农业科学—基础兽医学]