脂质体莪术醇联合顺铂抗人卵巢癌细胞作用和机制研究  

Effect and mechanism of liposome curcumol combined with cisplatin on human ovarian cancer cells

作　　者：张铭勋 张晨晨 蔡泽宇 宋永红 吴强[2] 王晶[1] Zhang Mingxun;Zhang Chenchen;Cai Zeyu(Dept of Obstetrics and Gynecology, The First Hospital of Anhui Medical University, Hefei 230022;Dept of Pathology,Anhui Medical University,Hefei 230032;Dept of Pathology,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第一附属医院妇产科,合肥230022 [2]安徽医科大学基础医学院病理教研室,合肥230032 [3]安徽医科大学第二附属医院病理科,合肥230601 [4]合肥工业大学,合肥230009

出　　处：《安徽医科大学学报》2021年第9期1430-1435,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省重点研究与开发计划项目(编号:202004j07020033)。

摘　　要：目的研究脂质体莪术醇(LC)联合顺铂抗人卵巢癌细胞的作用和机制。方法实验以卵巢癌细胞株SKOV3和HO8910为研究对象,以10μg/ml LC为基准,联合不同浓度的顺铂(0、2、4、8、16、32、64μg/ml)进行分组,MTT、Transwell和流式法分别检测各组细胞增殖、侵袭、迁移能力和凋亡率的变化,Western blot检测各组AKT、p-AKT和cleaved-Caspase8、9、3蛋白表达的变化。结果MTT结果显示,与单用顺铂或者LC组比较,联合用药组的肿瘤细胞增殖率降低(P<0.05),联合用药中顺铂对SKOV3的IC50为(12.67±2.03)μg/ml[单用顺铂的IC50:(38.28±5.98)μg/ml];联合用药中顺铂对HO8910的IC50为(10.55±1.55)μg/ml[单用顺铂的IC50:(26.41±2.30)μg/ml]。联合组(8μg/ml的顺铂+10μg/ml的LC)的卵巢癌细胞侵袭和迁移率均降低(P<0.05),凋亡率最高(P<0.05)。Western blot结果提示联合用药组卵巢癌细胞的p-AKT蛋白的表达水平降低(P<0.05),cleaved-Caspase8、9、3蛋白水平上升(P<0.05)。结论低剂量LC可以增强顺铂抑制人卵巢癌细胞增殖、迁移和侵袭能力,促进肿瘤细胞凋亡,联合用药可能通过PI3K/AKT通路促进卵巢癌细胞凋亡。Objective To study the effect and mechanism of liposomal curcumol(LC)combined with cisplatin on human ovarian cancer cells.Methods The experiment took ovarian cancer cell lines SKOV3 and HO8910 as the research objects,combined with different concentrations of cisplatin(0,2,4,8,16,32,64)μg/ml based on 10μg/ml LC for grouping.MTT,Transwell and flow cytometry assay were used to detect cell proliferation,invasion,migration and apoptosis ability respectively in each group.Western blot assay was used to detect AKT,p-AKT and cleaved Caspase 8,9,3 protein expression in each group.Results MTT results showed that compared with the cisplatin alone or LC group,the tumor cell proliferation rate of the combined drug group reduced(P<0.05),the IC50 of cisplatin to SKOV3 in the combination was(12.67±2.03)μg/ml vs the IC50 of cisplatin alone:(38.28±5.98)μg/ml,the IC50 of cisplatin to HO8910 in the combination was(10.55±1.55)μg/ml[vs IC50 of cisplatin alone:(26.41±2.30)μg/ml].In the combination group(8μg/ml cisplatin+10μg/ml LC),the invasion and migration rates of ovarian cancer cells reduced(P<0.05),and the apoptosis rate was the highest(P<0.05).Western Blot results showed that the expression level of p-AKT protein in ovarian cancer cells in the combined drug group decreased(P<0.05),and the protein levels of cleaved-Caspase8,9,3 increased(P<0.05).Conclusion Low-dose LC can enhance the ability of cisplatin to inhibit the proliferation,migration and invasion of human ovarian cancer cells,and promote tumor cell apoptosis.The combination of drugs may promote apoptosis of ovarian cancer cells through the PI3K/AKT pathway.

关 键 词：卵巢癌 莪术醇 顺铂 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]