胃癌TS表达及雷替曲塞腹腔给药对其影响研究  

Study on TS expression in gastric cancer and the effect of raltitrexed administration on it

作　　者：黄志国 胡孔旺[1] 李龙龙 冯振游 Huang Zhiguo;Hu Kongwang;Li Longlong(Dept of General Surgery,The First Affiliated Hospital of Anhui Medical University, Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院普外科,合肥230022

出　　处：《安徽医科大学学报》2021年第9期1458-1463,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省科技厅重点研究与开发项目(编号:202004j07020036);安徽省教育厅省级质量工程项目(编号:2019jxtd038);吴阶平医学基金会临床科研专项资助基金(编号:320.6750.19092-19)。

摘　　要：目的探讨胃癌中胸苷酸合成酶(TS)表达情况与临床病理因素、生存预后的关系及胃癌患者血液中TS浓度与雷替曲塞腹腔给药最适时间的关系。方法免疫组化检测80例胃癌及癌旁组织中TS表达情况;ELISA检测20例新鲜胃癌和癌旁组织中TS浓度及40例雷替曲塞腹腔给药患者给药前后血液中TS浓度;q-PCR检测20例新鲜冰冻胃癌及对应癌旁组织中TS mRNA的表达;腹腔化疗:术前2 d组腹腔穿刺给药,手术结束组关腹前给药。结果80例胃癌组织中TS的阳性率(48.75%,39/80)高于癌旁组织(23.75%,19/80),差异有统计学意义(P<0.001)。20例新鲜胃癌组织TS平均浓度(1.105×10^(-3)±0.177×10^(-3))μmol/L高于对应癌旁组织(0.811×10^(-3)±0.192×10^(-3))μmol/L,差异有统计学意义(P<0.001)。TS mRNA在20例新鲜胃癌组织(3.08±1.70)中相对表达量高于癌旁组织(1.02±0.23),差异有统计学意义(P<0.001);胃癌组织中TS表达与患者肿瘤分化程度相关(P<0.05),血液中TS表达与N分期及肿瘤分化程度相关(P<0.05);TS阴性胃癌患者生存率高于TS阳性患者(P<0.001),且TS还是胃癌预后的独立危险因素。雷替曲塞腹腔给药后血液中TS浓度在第2天降至最低值,术前2 d给药组无病生存率高于手术结束组(P<0.05)。结论TS在胃癌中阳性表达率增高,其表达与患者的肿瘤分化程度和生存预后相关。胃癌患者雷替曲塞腹腔给药后血液中TS浓度的变化可用于推测其腹腔给药最适给药时间,术前2 d给药为雷替曲塞腹腔给药的最佳时间。Objective To investigate the relationship between the expression of thymidine synthase(TS)in gastric cancer and clinicopathological factors,survival and prognosis,as well as the relationship between the blood TS concentration and the timing of intraperitoneal administration of raltitrexed in patients with gastric cancer.Methods Immunohistochemistry was used to detect TS expression in 80 cases of gastric cancer and adjacent tissues.Enzyme-linked immunosorbent assay(ELISA)was used to determine the concentration of TS in 20 cases of fresh gastric cancer and paracancer tissues,and the concentration of TS in blood before and after intraperitoneal administration of raltitrexed in 40 cases.q-PCR was used to detect TS mRNA expression in 20 cases of fresh frozen gastric cancer and its adjacent tissues.Intraperitoneal chemotherapy:Abdominal puncture was given to the group 2 d before surgery,and before abdominal closure was given to the group after surgery.Results The positive rate of TS in 80 gastric cancer tissues(48.75%,39/80)was higher than that in the adjacent tissues(23.75%,19/80),and the difference was statistically significant(P<0.001).The average TS concentration in the fresh gastric cancer tissues(1.105×10^(-3)±0.177×10^(-3))μmol/L was significantly higher than that in the adjacent gastric tissues(0.811×10^(-3)±0.192×10^(-3))μmol/L,and the difference was statistically significant(P<0.001).TS mRNA expression in 20 fresh gastric cancer tissues(3.08±1.70)was higher than that in adjacent gastric tissues(1.02±0.23),and the difference was statistically significant(P<0.001).The expression of TS in gastric cancer tissue was correlated with the degree of tumor differentiation(P<0.05),and the expression of TS in blood was correlated with N stage and the degree of tumor differentiation(P<0.05).The survival rate of patients with TS negative gastric cancer was higher than that of patients with TS positive gastric cancer(P<0.001),and TS was also an independent risk factor for the prognosis of gastric cancer.After intr

关 键 词：胃肿瘤 胸苷酸合酶 腹腔化疗 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]