新生鼠缺氧缺血性脑损伤远期行为学研究  

Long-term Behavioral Disorder after Hypoxic Ischemic Brain Damage in Newborn Mice

作　　者：林爱金 王洁琼 敖丽娟 陈茉弦 LIN Ai-jin;WANG Jie-qiong;AO Li-juan;CHEN Mo-xian(College of Rehabilitation,Kunming Medical University,Kunming,Yunnan 650500,China)

机构地区：[1]昆明医科大学康复学院,云南昆明市650500

出　　处：《中国康复理论与实践》2021年第8期908-912,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：云南省教育厅科学研究基金项目(No.2019Y0346)。

摘　　要：目的观察新生鼠缺氧缺血性脑损伤(HIBD)后运动、情绪和学习记忆能力的远期变化。方法10日龄C57BL/6小鼠50只分为对照组(n=20)和HIBD组(n=30)。HIBD组左侧颈总动脉结扎后缺氧45 min。2个月后,采用悬吊试验、明暗箱试验、高架十字迷宫试验、物体识别试验和Y迷宫试验进行评定。结果最终19只小鼠造模成功。与对照组相比,HIBD组悬吊试验评分降低(t=2.785,P<0.05);明暗箱试验潜伏期显著延长(t=-4.320,P<0.001),进入明箱的时间和次数减少(t>2.603,P<0.05);高架十字迷宫试验开臂停留时间显著缩短(t=4.576,P<0.001),闭臂停留时间明显延长(t=-3.287,P<0.01);物体识别试验旧物体停留时间延长(t=-2.116,P<0.05),物体识别指数下降(t=2.823,P<0.05);Y迷宫试验起始臂和新异臂停留时间缩短(t>2.191,P<0.05)。结论新生鼠HIBD后远期行为变化表现为运动功能、学习记忆功能下降,出现焦虑行为。Objective To observe the long-term behavioral changes in movement,emotion,and learning and memory of newborn mice with hypoxic ischemic brain damage(HIBD).Methods A total of 50 ten-day old newborn C57BL/6 mice were divided into control group(n=20)and HIBD group(n=30).The left common carotid artery was ligated in HIBD group and stayed in anoxic chamber for 45 min‐utes.All the mice were tested with suspension test,light/dark box test,elevated plus maze test,object recognition test and Y maze test two months after surgery.Results There were 19 mice modeled successfully.Compared with the control group,the suspension test scores de‐creased(t=2.785,P<0.05);the time of latency of light/dark box test increased(t=-4.320,P<0.001),the time and frequency in light box decreased(t>2.603,P<0.05);the time in open arm decreased(t=4.576,P<0.001)and the time in close arm increased(t=-3.287,P<0.01)for the elevated plus maze test;the time nearing old ob‐ject increased(t=-2.116,P<0.05)and object recognition index decreased(t=2.823,P<0.05)for object recog‐nition test;the time in the initial and novel arms decreased(t>2.191,P<0.05)for Y maze test in HIBD group.Conclusion The long-term disorders of behavior may include disabilities of motor,learning and memory,and disorder of anxiety,in newborn mice with HIBD.

关 键 词：缺氧缺血性脑损伤 运动 认知 焦虑 小鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]