巨噬细胞在β-TCP支架修复牙槽突裂中成骨作用的探讨  

Study on the osteogenic potential of macrophages in alveolar cleft repaired withβ-TCP scaffold

作　　者：单宇华 应惜裕 张晓宇 陈振琦[1] SHAN Yu-hua;YING Xi-yu;ZHANG Xiao-yu;CHEN Zhen-qi(Department of Orthodontics,Shanghai Ninth Peopled Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,Nalional Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔正畸科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海200011

出　　处：《中国口腔颌面外科杂志》2021年第4期315-319,共5页China Journal of Oral and Maxillofacial Surgery

摘　　要：目的:评价使用3D打印β磷酸三钙(β-TCP)支架复合骨髓干细胞(bone marrow stem cell,BMSC)修复牙槽突裂的成骨效果以及巨噬细胞在成骨中的作用。方法:将16只大鼠随机分为2组,将第1组8只大鼠双侧上颌第一磨牙拔除,形成4 mm×4 mm×3 mm的箱状骨缺损。8周后,一侧植入3D打印β-TCP复合干细胞支架,另一侧植入同样载有干细胞的空白支架。将第2组8只大鼠以相同方式造裂,两侧同时放置3D打印β-TCP复合干细胞支架,一侧注射氯膦酸盐脂质体清除巨噬细胞,另一侧注射对照脂质体。术后8周进行显微CT扫描和组织学分析,检测成骨效果。采用SAS 8.0软件包对数据进行统计学处理。结果:β-TCP支架移植侧BV/TV为(41.38±3.33)%,空白支架放置侧为(30.42±2.97)%,两者之间差异显著(P<0.05)。去除巨噬细胞后,BV/TV下降至(18.71±2.19)%,对照脂质体注射侧为(39.81±2.68)%,β-TCP支架放置侧和对照脂质体注射侧结果无统计学差异。结论:3D打印β-TCP支架复合骨髓干细胞有较好的成骨效果,巨噬细胞在β-TCP介导成骨过程中起到重要作用,去除巨噬细胞阻碍新骨形成。PRUPOSE:To study the osteogenic potential of 3 D printedβ-TCP scaffold combined with bone mar row stem cells(BMSCs)repairing alveolar cleft and the role of macrophages in osteogenesis.METHODS:Bilateral maxillary first molars were extracted in the first eight rats to form a box shaped bone defect in the size of 4 mm×4 mm×3 mm.3 D printedβ-TCP scaffold with BMSCs was implanted on one side and control scaffold was implanted on the other side eight weeks later.In the second group,3 D printedβ-TCP scaffold with BMSC was placed on both sides at the same time,and macrophages were removed on one side.Eight weeks after operation,Micro-CT scanning and histological analysis were performed to detect bone formation.SAS 8.0 software was performed for statistical analysis.RESULTS:BV/TV ratio was(41.38±3.334)%inβ-TCP group and(30.42±2.964)%in the control group(P<0.05).BV/TV ratio was(18.71±2.193)%in macrophage-depletion group and(39.81%±2.678)%in the control group.There was no significant difference between the two groups with the sameβ-TCP side.CONCLUSIONS:BMSCs combined with 3 D printedβ-TCP scaffold has satisfying osteogenic ability,and macrophages play an important role inβ-TCP mediated osteogenesis.The depletion of macrophages hinders new bone formation.

关 键 词：磷酸三钙 牙槽突裂 骨免疫学 骨替代物 

分 类 号：R782.2[医药卫生—口腔医学]