Chromosome-level genome of Himalayan yew provides insights into the origin and evolution of the paclitaxel biosynthetic pathway  

作　　者：Jian Cheng Xiao Wang Xiaonan Liu Xiaoxi Zhu Zihe Li Huanyu Chu Qian Wang QianQian Lou Bijun Cai Yiqun Yang Xiaoyun Lu Kai Peng Dingyu Liu Yuwan Liu Lina Lu Huan Liu Ting Yang Qijin Ge Chengcheng Shi Guichun Liu Zhiwei Dong Xun Xu Wen Wang Huifeng Jiang Yanhe Ma 

机构地区：[1]Key Laboratory of Systems Microbial Biotechnology,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China [2]National Center of Technology Innovation for Synthetic Biology,Tianjin 300308,China [3]State Key Laboratory of Agricultural Genomics,BGI-Shenzhen,Shenzhen,China [4]State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming,Yunnan 650223,China [5]Jiaxing Synbiolab Biotechnology Co.,Ltd.,Jiaxing 314006,China [6]University of Chinese Academy of Sciences,Beijing 100049,China [7]School of Ecology and Environment,Northwestern Polytechnical University,Xi'an,Shaanxi 710072,China

出　　处：《Molecular Plant》2021年第7期1199-1209,共11页分子植物（英文版）

基　　金：the National Key R&D Program of China(2020YFA0908000);National Science Fund for Excellent Young Scholars(31922047);Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(No.TSBICIP-KJGG-002);the China Postdoctoral Science Foundation(No.2019M661032)。

摘　　要：Taxus,commonly known as yew,is a well-known gymnosperm with great ornamental and medicinal value.In this study,by assembling a chromosome-level genome of the Himalayan yew(Taxus wallichiana)with 10.9 Gb in 12 chromosomes,we revealed that tandem duplication acts as the driving force of gene family evolution in the yew genome,resulting in the main genes for paclitaxel biosynthesis,i.e.those encoding the taxadiene synthase,P450s,and transferases,being clustered on the same chromosome.The tandem duplication may also provide genetic resources for the nature to sculpt the core structure of taxoids at different positions and subsequently establish the complex pathway of paclitaxel by neofunctionalization.Furthermore,we confirmed that there are two genes in the cluster encoding isoenzymes of a known enzyme in the paclitaxel biosynthetic pathway.The reference genome of the Himalayan yew will serve as a platform for decoding the complete biosynthetic pathway of paclitaxel and understanding the chemodi-versity of taxoids in gymnosperms.

关 键 词：TAXUS genome sequencing tandem duplication paclitaxel biosynthetic pathway gene cluster 

分 类 号：Q942[生物学—植物学]