近交系小型猪线粒体基因组分析和家猪系统进化关系分析  

Analysis of Inbred Miniature Pig Mitochondrial Genome and Phylogenetic Study of Domestic Pigs

作　　者：高也凡 陈笑寒 邹辉[1] 郭亚芬[1] 黄艳娜[1] 蒋钦杨[1] Gao Yefan;Chen Xiaohan;Zou Hui;Guo Yafen;Huang Yanna;Jiang Qinyang(College of Animal Science and Technology,Guangxi University,Nanning,530004)

机构地区：[1]广西大学动物科学技术学院,南宁530004

出　　处：《基因组学与应用生物学》2021年第2期531-540,共10页Genomics and Applied Biology

基　　金：广西大学“大学生创新创业计划”项目(201610593253)资助。

摘　　要：广西巴马小型猪是长期近交培育而成的实验小型猪,遗传稳定。本研究拟通过线粒体基因组(mitochondrial DNA,mtDNA)分析来了解近交系的遗传背景,并从mtDNA角度进一步认识中国本土猪种的系统进化关系。实验设计29对特异性引物,PCR扩增和产物测序,拼接出广西巴马小型猪近交系7个世代猪的mtDNA序列;用42个品种猪的mtDNA D-loop区和基因编码区序列分布构建系统进化树。结果表明,广西巴马小型猪的mtDNA大小为16761 bp,碱基组成、基因结构与其他猪种相似;近交系7个世代个体mtDNA发现有46个碱基差异位点,变异率为0.28%,mtDNA序列同源性在99.9%以上,表明该近交系母源单一;基于mtDNA序列构建的系统进化树不能清晰地区分地方品种猪之间的遗传关系。研究结果进一步表明广西巴马小型猪近交系的遗传背景单一,中国本土品种猪的母系关系复杂。Guangxi Bama miniature pig is a kind of experimental miniature pig cultivated by long-term inbreeding and is genetically stable.This study intends to understand the genetic background of inbred line through mitochondrial genome(mitochondrial DNA,mtDNA)analysis,and further understand the phylogenetic relationship of local pig breeds from mtDNA.This experiment designed 29 pairs of specific primers,and used PCR amplification and product sequencing to splice out mtDNA sequences of 7 generations of Bama miniature pig inbred lines;the phylogenetic trees were constructed with the mtDNA D-loop region and gene coding region sequence of 42 breeds pigs.The results showed that the mtDNA size of Bama miniature pig was 16761 bp,its base composition and gene structure were similar to those of other pig breeds.There were 46 base difference sites found in the mtDNA of seven generations of inbred line,and the base mutation rate was 0.28%.The mtDNA sequence homology was above 99.9%,indicating that the maternal source of the inbred line was single.The phylogenetic tree constructed based on the mtDNA sequence could not clearly distinguish the genetic relationship among the local breed pigs.These results further indicated that the genetic background of Bama miniature pig inbred line was single,and the maternal relationship of the Chinese pigs was complicated.

关 键 词：广西巴马小型猪近交系 线粒体基因组 系统进化 

分 类 号：S828[农业科学—畜牧学]