Molecular evolution and genetic diversity analysis of SFTS virus based on next-generation sequencing  

作　　者：Aqian Li Lin Liu Wei Wu Yang Liu Xiaoxia Huang Chuan Li Di Liu Jiandong Li Shiwen Wang Dexin Li Mifang Liang 

机构地区：[1]Department of Viral Hemorrhagic Fever,National Institute for Viral Disease Control and Prevention,China CDC,Beijing 102206,China [2]Suzhou Institute of Systems Medicine,Suzhou 215123,China [3]Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China [4]China CDC-WIV Joint Research Center for Emerging Diseases and Biosafety,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan 430071,China

出　　处：《Biosafety and Health》2021年第2期105-115,共11页生物安全与健康（英文）

基　　金：supported by the National Major Science and Technol-ogy Project of China(2018ZX10711001,2016ZX10004222-002)。

摘　　要：SFTS virus(SFTSV)is a novel bunyavirus,which was discovered as the etiological agent of severe fever with thrombocytopenia syndrome(SFTS)in China in 2009,and was now prevalent in at least 25 provinces in China.SFTS was subsequently identified in South Korea and Japan in 2012.To explore themolecular evolution and genetic characteristics of this newly identified pathogen,we reported 72 whole genome sequences of SFTSV,and built a dataset of SFTSV genome sequences containing 292 L-segment,302 M-segment and 502 S-segment.We clearly divided SFTSV into six genotypes,Genotype A-F.It was found that genotype F was the dominant epidemic genotype of Japan,South Korea,and Zhejiang province of China.The coalescent analysis supported that SFTSV originated in the early 18th century from Zhejiang province,and Genotype F was the most primitive one.Henan,Hubei,and Anhui provinces which are located in Dabie Mountain area weremainly epidemic of Genotype A,which emerged relatively late but distributed widely.A total of 37 recombination events were identified,making SFTSV with a high recombination frequency(L segment 5.1%,Msegment 3.6%,S segment 0.8%)among negative-strand segmented RNA viruses.It was identified that 19 reassortant strains belonged to 12 reassortment forms of SFTSV genome containing 6 newly identified forms.The reassortment virus and recombination in tick were both found for the first time.We also found many of genotype-specific mutation sites,7 of which could be considered as potential molecular marker for genotype classification.This study promoted a more comprehensive understanding of the phylogeny and origin,and the genetic diversity of SFTSV,and it could help the studies of other newly discovered tick-borne bunyavirus as reference data and research ideas.

关 键 词：SFTS virus(SFTSV) Next-generation sequencing GENOTYPE Reassortment and recombination COALESCENT 

分 类 号：R373.3[医药卫生—病原生物学]