抑郁症模型小鼠肠道菌群的微生物生态学研究  

Study on the Microbial Ecology of Intestinal Microflora in Depression Model Mice

作　　者：张雪 杨树林 邢一凡 张盛帆 李文婧 王宪昊 曲星雯 杨帆 ZHANG Xue;YANG Shulin;XING Yifan;ZHANG Shengfan;LI Wenjing;WANG Xianhao;QU Xingwen;YANG Fan(Shandong First Medical University&Shandong Academy of Medical Sciences,Shandong Tai'an 271016;The Second Affiliated Hospital of Shandong First Medical University,Shandong Tai'an 271016)

机构地区：[1]山东第一医科大学(山东省医学科学院)公共卫生学院,山东泰安271016 [2]山东第一医科大学第二附属医院,山东泰安271000

出　　处：《生物化工》2021年第4期19-24,共6页Biological Chemical Engineering

基　　金：山东省大学生创新创业训练计划(S201910439132);山东第一医科大学大学生计划课题(201901006)。

摘　　要：目的:基于16S rDNA宏基因分类测序分析,通过检测抑郁症造模成功小鼠的粪便标本,探讨抑郁症小鼠肠道菌群的微生物生态学特点。方法:选择4周龄SPF级雌性小鼠,并随机分组;通过皮下注射苯甲酸雌二醇造模;记录实验组与对照组小鼠实验过程体重、饮食、学习记忆能力变化并进行统计分析,确定造模效果;采集造模成功小鼠与正常对照小鼠粪便标本,进行粪便菌群宏基因组检测与分析。结果:小鼠造模后呈现明显的抑郁样表现,主要表现在体重增加速度减慢、饮食欲望减退、学习记忆能力明显下降以及菌群多样性降低,g_unclassified_Enterobacteriaceae和g_Roseburia两个属在实验组与对照组间差异显著(P<0.05)。结论:小鼠长期处于高雌激素的病理状态会导致抑郁,并且抑郁症小鼠相较正常小鼠存在肠道菌群紊乱现象。Objective:Based on 16S rDNAacer gene classification and sequencing analysis,the fecal samples of mice with depression were detected to explore the microbial ecology characteristics of intestinal microflora of mice with depression.Methods:Female mice of SPF grade at 4 weeks of age were selected and randomly assigned.The model was made by subcutaneous injection of estradiol benzoate;The experimental results of body weight,diet,learning and memory ability of experimental group and control group were recorded,and statistical analysis was made to determine the modeling effect;Fecal specimens of mice with successful modeling and normal control were collected and sent for metagenomic detection and analysis of fecal flora.Results:The mice show obvious depression-like performance after modeling,mainly manifested in slower weight gain,decreased appetite for food,significantly decreased learning and memory ability,and decreased diversity of bacteria.Compared with normal mice,g_Unclassified_Enterobacteriaceae and g_Roseburia were significantly different between the experimental group and the control group(P<0.05).Conclusion:Long-term exposure to high estrogen levels in mice leads to depression,and the intestinal flora of depressed mice is disturbed compared with normal mice.

关 键 词：苯甲酸雌二醇 抑郁症造模 肠道菌群 宏基因测序 

分 类 号：R749.4[医药卫生—神经病学与精神病学]