氧化应激调控在CHO细胞培养中的应用  

Applications of Oxidative Stress Regulation in CHO Cell Culture

作　　者：孔倩倩 陈飞 白晓雨 张娟[1] KONG Qianqian;CHEN Fei;BAI Xiaoyu;ZHANG Juan(School of Life Science and Technology,China Pharmaceutical University,Jiangsu Nanjing 210009;Cell Culture Process Development,WuXi Biologics,Shanghai 200131)

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京211100 [2]上海药明生物技术有限公司细胞培养工艺开发部,上海200131

出　　处：《生物化工》2021年第4期147-153,共7页Biological Chemical Engineering

摘　　要：抗体,作为应用广泛的一类蛋白质药物,主要通过大规模培养中国仓鼠卵巢(Chinese hamster ovary,CHO)细胞生产。但在CHO细胞培养过程中,细胞高水平代谢伴随着大量活性氧(Reactive Oxygen Species,ROS)生成,环境压力也可促进ROS产生。当抗氧化防御系统难以与其抗衡时将出现氧化应激,不利于细胞生长代谢和生产。本文综述了CHO细胞培养中氧化应激调控的三类工程学方法,即基因工程、抗氧化型添加剂和培养过程控制的应用,旨在为细胞培养时调控氧化还原平衡提供思路。As a widely used therapeutic protein drugs,antibodies are mainly produced by Chinese hamster ovary(CHO)cell culture in large scales.In culture process,elevated reactive oxygen species(ROS)are produced along with enhanced cell metabolism.Besides,intracellular ROS will be formed under some stress conditions.The imbalance between intracellular ROS and antioxidant defense system will impose oxidative stress and be harmful to cell growth and productivity.Herein,three major engineering methods to alleviate oxidative stress in CHO cell culture,including cell engineering,antioxidants addition and culture process control,will be reviewed,aiming to provide strategies to regulate redox homeostasis during cell culture.

关 键 词：中国仓鼠卵巢细胞 活性氧 氧化应激 抗氧化剂 

分 类 号：R363[医药卫生—病理学]