典型红壤水稻土剖面细菌和真菌分子生态网络特征研究  

Molecular Ecological Network of Bacteria and Fungi in Paddy Soil Profile of Typical Red Soil

作　　者：黄兰婷 倪浩为 李新宇[3] 孙波[2] 梁玉婷[2] HUANG Lanting;NI Haowei;LI Xinyu;SUN Bo;LIANG Yuting(School of Environmental and Safety Engineering,Changzhou University,Changzhou,Jiangsu 213164,China;State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 201008,China;Key Laboratory of Pollution Ecology and Environmental Engineering,Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110164,China)

机构地区：[1]常州大学环境与安全工程学院,江苏常州213164 [2]土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京210008 [3]污染生态与环境工程重点实验室(中国科学院应用生态研究所),沈阳110164

出　　处：《土壤学报》2021年第4期1018-1027,共10页Acta Pedologica Sinica

基　　金：国家自然科学基金项目(41430856,41622104);国家重点研发计划项目(2016YFD0200309)资助。

摘　　要：土壤表层及深层的微生物互作对土壤养分循环和物质转化等具有重要意义。为揭示典型红壤水稻土剖面微生物分子生态网络特征,通过16S rRNA和18S rRNA高通量测序技术,运用CoNet分析方法,构建上层(0~20 cm)、中层(20~60 cm)和下层(60~100 cm)土壤细菌、真菌分子生态网络。结果表明,土壤全碳和全氮含量、细菌和真菌的丰富度沿土壤剖面显著降低(P<0.05)。微生物网络拓扑参数,如连通度、群聚系数和网络密度等均明显增加,表明微生物互作随深度增加而增强。与细菌和真菌界内网络互作特征相反,界间的网络互作随深度减弱。进一步利用随机森林模型和方差分解分析,表明土壤碳氮是影响微生物分子生态网络的关键因素;沿土壤剖面碳对微生物互作的贡献逐渐增加,由上层的3.58%增加到下层的32.67%。【Objective】Soil environmental factors are highly spatially heterogeneous,especially soil pH,nutrient availability,soil temperature and soil microbes,which vary with soil depth,and hence affect the distribution of soil microbial communities.Soils in deep soil layers may have microbial communities that have adapted to the environments of deep soil layers,and consequently are different in structure from those in topsoil layers.Microbial communities decrease in biomass and diversity with depth in the soil profile,which alters structures of the soil microbial communities and affects their functions.Microbes in deep soil play an important role in soil formation,biogeochemical reactions and pollutant degradation.So deep soil has significant influences on quality indices of the soil and hence productivity of the vegetation on the surface.Interactions between species of the microbes may be more important than richness and diversity to ecosystem function of the microbial community,especially in complex ecosystems.Interactions between microbial communities are a part of the microbial network of a terrestrial ecosystem,and the basis of biochemical cycles.Ecological network analysis is a new analysis method to visualize interactions between microbial communities and explore co-existence patterns of the species in microhabitats and their main influencing factors.Network analysis can be used to explore mechanisms of microbial interactions driving biogeochemical coupling of important elements in soil,and hence is an important means to improve service functions of a soil ecosystem.However,most of the previous studies on soil microbial communities focused on those in the topsoil(0-20 cm)layer or on abundance of microbial species.Therefore,the information available in the literature about potential relationships between microbial interactions and soil depth,and their determining environmental factors.【Method】After late rice was harvested,soil samples were collected from a paddy field in Yingtan(116°54′E,28°13′N,34-62

关 键 词：土壤深度 网络分析 细菌-真菌共现网络 界内互作 界间互作 

分 类 号：X172[环境科学与工程—环境科学]