掺锶TiO_(2)纳米管涂层种植体的促成骨性能研究  

Osteogenic properties of strontium doped TiO_(2) nanotube coating implants

作　　者：朱晔 温黎明 李任 毕文娟 于津建 郑天霞 戚孟春 Zhu Ye;Wen Liming;Li Ren(School of Stomatology, North China University of Science And Technology,Tangshan 063000)

机构地区：[1]华北理工大学口腔医学院,唐山063000

出　　处：《安徽医科大学学报》2021年第8期1185-1190,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81600844、81270965)。

摘　　要：目的探讨掺锶TiO_(2)纳米管涂层种植体的促成骨性能。方法通过阳极氧化法在纯钛表面制备TiO_(2)纳米管,经由水热反应将锶掺入TiO_(2)纳米管中,构建新型种植体涂层。通过发射扫描电镜、X射线衍射仪、原子力显微镜、表面接触角测量仪、电感耦合等离子体光谱仪对涂层表面微观形貌、元素分布、晶体相、亲水性和涂层中Sr2+释放情况进行检测;通过体外细胞学实验评价涂层上MC3T3-E1的细胞相容性和促成骨活性;通过Micro-CT影像学检测来分析掺锶TiO_(2)纳米管种植体在体内的促成骨效果。结果Sr被成功地掺入TiO_(2)纳米管中,并主要以钛酸锶的形式存在。掺Sr后涂层表面粗糙度相较于TiO_(2)纳米管无显著变化,亲水性显著上升;TiO_(2)涂层中Sr的掺入促进了细胞黏附、增殖,提高了碱性磷酸酶活性;掺锶TiO_(2)纳米管涂层种植体使大鼠种植体周围骨体积分数显著增加。结论掺锶TiO_(2)纳米管涂层种植体显示出良好的细胞相容性和成骨活性,并能够促进骨生成。Objective To explore osteogenic properties of strontium doped TiO_(2) nanotube implants.Methods TiO_(2) nanotubes were prepared on the surface of pure titanium by anodic oxidation,strontium was doped into TiO_(2)nanotubes by hydrothermal reaction to construct a new implant coating.The coating’s surface morphology,element distribution,crystal phase,hydrophilicity and the release concentration of Sr2+in the coating were detected by scanning emission electron microscopy,X-ray diffraction,atomic force microscopy,surface contact angle measurement instrument and inductively coupled plasma spectrometer.The cytocompatibility and osteogenic activity of MC3T3-E1 on the coating were evaluated by cytological test in vitro,and the osteogenic effect of strontium doped TiO_(2) nanotube implants in vivo was analyzed by Micro-CT.Results Sr was successfully incorporated into TiO_(2) nanotube,mainly in the form of strontium titanate.The surface roughness of the coating had no significant change compared with that of TiO_(2) nanotube,and the hydrophilicity of the coating increased significantly;the strontium doped TiO_(2) nanotube coating promoted the cell adhesion and proliferation,and increased the alkaline phosphatase activity.The strontium doped TiO_(2) nanotube coating implant significantly increased the bone volume fraction around the implant.Conclusion Sr doped TiO_(2) nanotube coating implants show good biocompatibility and osteogenic activity,which can promote bone formation.

关 键 词：锶 TiO_(2)纳米管 种植体 成骨能力 

分 类 号：R782[医药卫生—口腔医学]