MTAN用于抑制血链球菌、具核梭杆菌、牙龈卟啉单胞菌混合菌的体外研究  

Vitro study of MTAN to inhibit the mixed bacteria solutions of S.sanguinis,F.nucleatum and P.gingivalis

作　　者：周琳[1] 蔡文静 杨慧 吴梦楠 郑营雪 Zhou Lin;Cai Wenjing;Yang Hui(Dept of Prosthodontics,Henan Provincial Stomatological Hospital of The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000;Dept of Prosthodontics,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000;Dept of Prosthodontics,Zhengzhou City Stomatology Hospital,Zhengzhou 450000)

机构地区：[1]郑州大学第一附属医院河南省口腔医院口腔修复科,郑州450000 [2]郑州大学第一附属医院口腔修复科,郑州450000 [3]郑州市口腔医院口腔修复科,郑州450000 [4]新疆医科大学第一附属医院口腔修复科,乌鲁木齐830000 [5]安阳市口腔医院口腔修复科,安阳455000

出　　处：《安徽医科大学学报》2021年第8期1328-1331,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:U1404813)。

摘　　要：研究Nisin粉、柠檬酸、吐温-80混合物(MTAN)和乙二胺四乙酸(EDTA)(MTAN+EDTA)对血链球菌、具核梭杆菌、牙龈卟啉单胞菌双混合及三混合菌液的抑制作用。用含有不同乳酸链球菌素(Nisin)浓度的MTAN、MTAN+EDTA各处理血链球菌、具核梭杆菌、牙龈卟啉单胞菌双混合及三混合菌液,OD值法测最低抑菌浓度(MIC)、最小杀菌浓度(MBC)。结果示MTAN对各混合菌液均可抑制,浓度增加,抑制增强,浓度≥250μg/ml时可杀菌;不同浓度的Nisin组MTAN+EDTA对各混合菌液均可抑制,浓度≥2.5μg/ml时可杀菌。因此,MTAN作用于3种细菌的各菌液后均有显著抗菌作用,加入EDTA后,抗菌作用增强。In order to study the inhibition effect of MTAN(a mixture of Nisin,acid,and detergent)and MTAN+(ethylene diamine tetraacetic acid,EDTA)on S.sanguinis,F.nucleatum and P.gingivalis paired mixed bacteria solutions and three-in-one mixed bacteria solution,MTAN and MTAN+EDTA with different Nisin concentrations were used to treat S.sanguinis,F.nucleatum and P.gingivalis paired mixed bacteria solutions and three-in-one mixed bacteria solution respectively,and the OD method was adopted to measure MIC and MBC.The results showed that MTAN had inhibitory effect on all mixed bacteria solutions.With the increase of concentration,the inhibitory effect was enhanced.When Nisin concentration was≥250μg/ml,bacteria could be killed.MTAN+EDTA with different concentrations of Nisin inhibited all mixed bacterial solutions,and when the concentration of Nisin≥2.5μg/ml,bacteria could be killed.Therefore after MTAN acts on all the mixed bacteria solutions,it has obvious bacteriostatic effect,and this effect is obviously improved with the addition of EDTA.

关 键 词：乳酸链球菌素 MTAN EDTA 混合菌液 

分 类 号：R783.6[医药卫生—口腔医学]