泪腺良性淋巴上皮病变基因网络分析及关键枢纽基因鉴定  

Identification of hub genes of benign lymphoepithelial lesion with weighted gene co-expression network analysis

作　　者：张黎黎[1] 刘文辉 刘小静 丁岩[1] 张琪[1] 刘瑜[2] 刘林嶓[1] ZHANG Lili;LIU Wenhui;LIU Xiaojing;DING Yan;ZHANG Qi;LIU Yu;LIU Linbo(Department of Plastic and Reconstructive Surgery,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China;Department of Ophthalmology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院整形外科,河南郑州450052 [2]郑州大学第一附属医院眼科,河南郑州450052

出　　处：《肿瘤基础与临床》2021年第4期290-293,共4页journal of basic and clinical oncology

摘　　要：目的构建泪腺良性淋巴上皮病变(BLEL)基因共表达网络,明确基因网络中的关键枢纽基因。方法从Gene Expression Omnibus数据库下载基因芯片数据,求差异基因,利用差异基因构建加权基因共表达网络,明确网络中与疾病最相关的基因模块及其关键枢纽基因。结果共得到14878个差异表达基因,构建的基因共表达网络中共包括12个基因模块,其中turquoise模块与BLEL相关系数为-0.94,对应的P<0.001,模块内排名最高的关键枢纽基因依次是MAP1B、DOCK6、RGS5、ESAM和PDE5A。结论本研究构建了BLEL中的加权基因共表达网络,加深了我们对BLEL发病机制的理解,这些关键枢纽基因也可作为治疗BLEL的新的靶点。Objective To construct weighted gene co-expression network of benign lymphoepithelial lesion(BLEL),and to identify the hub genes.Methods Microarray data were downloaded from Gene Expression Omnibus.Weighted gene co-expression network was constructed with differentially expressed genes.Module mostly related to BLEL and its hub genes in the network were identified.Results Weighted gene co-expression network containing 12 modules was constructed with 14878 differentially expressed genes.Module turquoise was most related to BLEL with correlation coefficient-0.94 and P<0.001.Top hub genes in the modules were MAP1B,DOCK6,RGS5,ESAM and PDE5A.Conclusion The present study revealed weighted gene co-expression network of BLEL which could deepen our understanding and hub genes identified may serve as potential treatment targets.

关 键 词：泪腺良性淋巴上皮病变 基因芯片 加权基因共表达网络分析 关键枢纽基因 

分 类 号：R730[医药卫生—肿瘤]