Identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge, Guyana  

作　　者：Ji Wang Weimin Zhou Hua Ling Xiaoping Dong Yi Zhang Jiandong Li Yong Zhang Jingdong Song William J.Liu Yang Li Ruiqing Zhang Wei Zhen Kun Cai Shuangli Zhu Dongyan Wang Jinbo Xiao Yigang Tong Wenli Liu Lihua Song Wei Wu Yang Liu Xiang Zhao Ruihuan Wang Sheng Ye Jing Wang Roujian Lu Baoying Huang Fei Ye Wenwen Lei Rongbao Gao Qi Shi Cao Chen Jun Han Wenbo Xu George F.Gao Xuejun Ma Guizhen Wu 

机构地区：[1]National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China [2]Beijing Advanced Innovation Center for Soft Matter Science and Engineering(BAIC-SM),College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China [3]Chongqing Center for Disease Control and Prevention,Chongqing 400042,China [4]Chongqing Public Health Medical Center,Chongqing 400035,China [5]Hunan Provincial Center for Disease Control and Prevention,Changsha 410005,China [6]Hebei Medical University,Shijiazhuang 050031,China [7]Center for Biosafety Mega-science,Chinese Academy of Science,Wuhan 430200,China

出　　处：《Biosafety and Health》2019年第3期150-154,共5页生物安全与健康（英文）

基　　金：This work was supported by grants from the China MegaProjects for Infectious Disease(2018ZX10713-002,2018ZX10711001,2017ZX10104001,and 2017ZX10302301-004-002).

摘　　要：Here,we report the identification of Histoplasma causing an unexplained disease cluster in Matthews Ridge,Guyana.In March 2019,14 employees of Chongqing Bosai Mining Company,China,working in a manganese mining of Guyana,had unexplained fever,and two of them died.We obtained lung and brain tissues as well as the blood samples from the two deceased cases(patient No.1 and 2),and bronchoscopy lavages and cerebrospinal fluid samples from one severe case(patient No.3),respectively.All samples were tested by pathological examination,high-throughput sequencing,and real-time PCR.Pathological detection showed the presence of spore-like structures in the lung tissue of patient No.1,indicating a fungal infection in this patient.Nanopore sequencing identified the existing of H.capsulatum in the lung tissue sample within 13 h.Next-generation sequencing identified specific fragments of H.capsulatum in all of the samples tested(lung,brain and blood serum from the deceased cases,and plasma from the severe case).Real-time PCR assays did not reveal any viral infection related to transmission from bat feces.We conclude that H.capsulatum was the causative pathogen of this disease cluster based on epidemiologic,clinical,pathological and nucleic acid evidence.

关 键 词：HISTOPLASMA FEVER Histochemical examination Nanopore high-throughput sequencing Real-time PCR 

分 类 号：R73[医药卫生—肿瘤]