Effects of Cryopreservation on Human Sperm Glycocalyx  

作　　者：Yan-Cheng Wu Ai-Jie Xin Hui Lu Hua Diao Li Cheng Yi-Hua Gu Bin Wu Sheng-Ce Tao Zheng Li Hui-Juan Shi Yong-Lian Zhang 

机构地区：[1]Department of Applied Biology,State Key Laboratory of Bioreactor Engineering,School of Biotechnology,East China University of Science and Technology,Shanghai 200237,China [2]Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases,Shanghai Ji Ai Genetics and IVF Institute,Obstetrics and Gynecology Hospital,Fudan University,Shanghai 200011,China [3]Department of Allergy,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200001,China [4]Department of Reproductive Pharmacology,NPFPC Key Laboratory of Contraceptives and Devices,Shanghai Institute of Planned Parenthood Research,Shanghai 200032,China [5]Shanghai Center for Systems Biomedicine,Shanghai Jiao Tong University,Shanghai 201100,China [6]Department of Andrology,Urologic Medical Center,Shanghai General Hospital,Shanghai Jiao Tong University,Shanghai 200080,China [7]Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Reproductive and Developmental Medicine》2017年第4期233-238,共6页生殖与发育医学（英文版）

基　　金：We thank the financial support from National Natural Science Foundation of China(81401252);MerckSerono China Research Fund for Fertility Experts.

摘　　要：Background:To study the effects of cryopreservation on human sperm glycocalyx.Methods:The lectin binding profilings of sperm after freeze-thaw were compared by lectin microarray.Results:CryoSperm^(TM) and direct fumigation were confirmed to be the optimized cryoprotectant and method by comparing the sperm recovery rate.In 91 lectins,33 lectins were significantly changed after sperm cryopreservation.Among them,9 lectins greatly decreased and 24 lectins mainly increased.The binding signals of MAA,PSA,ABA,and AIA were verified by FACS,and the results were consistent with that of lectin microarray.Conclusions:Sperm glycocalyx had significant changes after cryopreservation.The sialic acid,playing an important role in protecting sperm,was greatly lost,which exposed the inner carbohydrates.Thus,the glycocalyx damage due to the cryopreservation might be one of the reasons for low sperm fertility.

关 键 词：Cryopreservation Damage GLYCOCALYX Lectin Microarray SPERM 

分 类 号：R318.52[医药卫生—生物医学工程]