UvSMEK1,a Suppressor of MEK Null,Regulates Pathogenicity,Conidiation and Conidial Germination in Rice False Smut Fungus Ustilaginoidea virens  

作　　者：Yu Junjie Yu Mina SONG Tianqiao CAO Huijuan Yong Mingli Pan Xiayan Qi Zhongqiang Du Yan Zhang Rongsheng Yin Xiaole Liang Dong Liu Yongfeng 

机构地区：[1]Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China [2]School of the Environment and Safety Engineering,Jiangsu University,Zhenjiang 212013,China

出　　处：《Rice science》2021年第5期457-465,I0018,I0019,共11页水稻科学（英文版）

基　　金：supported by the National Key Research and Development Project in China(Grant No.2016YFD200805);National Natural Science Foundation of China(Grant Nos.31301624 and 31571961).

摘　　要：Rice false smut,which is caused by Ustilaginoidea virens,is an emerging disease of rice spikelets in rice-growing areas worldwide.However,the infection mechanism of U.virens on rice spikelets is still unclear.Here,we characterized a suppressor of mitogen-activated protein kinase kinase or ERK kinase(MEK)null(UvSMEKI)in U.virens that is conserved among filamentous fungi.Compared with wild type U.virens strain P-1,UvSMEKI deletion mutants were defective in pathogenicity and conidial germination.In addition,conidiation of UvSMEKI deletion mutants was significantly reduced on yeast extract tryptone(YT)plates,but inc「eased in YT broth compared with the wild type.Compared with UvSMEKI expression level during the vegetative mycelia and conidiation stages,UvSMEKI dramatically increased during infection of rice florets.Surprisingly,the UvSMEKI deletion mutants exhibited higher tolerance to H_(2)O_(2) and NaCl.In summary,presented evidence suggested that UvSMEKI positively regulated pathogenicity,conidial germination and conidiation in YT broth,and negatively regulated conidiation on YT medium and tolerance to oxidative and osmotic stresses.The results enhanee our understanding of the regulatory mechanism of pathogenicity of U.virens,and present a potential molecular target for blocking rice infection by U.virens.

关 键 词：SUPPRESSOR MEK null Ustilaginoidea virens PATHOGENICITY conidial germination CONIDIATION 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]