侵蚀-沉积作用对黄土高原土壤中固碳细菌群落分布特征的影响  

The influence of erosion-sedimentation on the distribution characteristics of carbon-fixing bacterial communities in the Loess Plateau

作　　者：彭诗阳 钟仁华 吴岸松 蔡红春 何勇 张燕茹 PENG Shiyang;ZHONG Renhua;WU Ansong;CAI Hongchun;HE Yong;ZHANG Yanru(Hunan Xinheng Environmental Technology Co.Ltd.,Changsha 410000,Hunan,China;Hunan Xinheng Ecological Research Co.Ltd.,Yiyang 413002,Hunan,China;Hunan Academy of Forestry,Changsha 410004,Hunan,China)

机构地区：[1]湖南鑫恒环境科技有限公司,湖南长沙410000 [2]湖南鑫恒生态研究院有限公司,湖南益阳413002 [3]湖南省林业科学院,湖南长沙410004

出　　处：《湖南林业科技》2021年第4期1-9,共9页Hunan Forestry Science & Technology

基　　金：湖南省林业科技创新专项(XLK201901);湖南省林业科技创新专项(XLK201908)。

摘　　要：针对黄土高原土壤固碳细菌对侵蚀-沉积的响应规律尚不清晰这一科学问题,在甘肃省罗峪沟流域的主要分支—桥子沟流域选择包括未开垦坡面的侵蚀点和沉积点、刺槐坡面的侵蚀点和沉积点共4个具有代表性的采样点,以土壤样品中有固碳功能基因cbb L的自养细菌为研究对象,采用高通量测序技术研究固碳细菌群落的多样性、分布特征和结构差异。结果显示:刺槐坡面固碳细菌多样性和丰富度明显低于未开垦坡面的。测序数据共鉴定出21个携带cbb L固碳功能基因的自养细菌属,这些属隶属于3个细菌门,包括芽单胞菌门、变形菌门和放线菌门。主坐标分析和聚类分析表明,同一坡面下侵蚀点和沉积点的自养固碳细菌群落构成相似。相关性分析表明,土壤全钾、全氮和pH值与细菌群落组成的变化有很高的相关性。The response of soil carbon-fixing bacteria to erosion-deposition in the Loess Plateau is still unclear.Therefore,the main branch of the Luoyugou watershed in Gansu Province,Qiaozigou watershed,selected four representative sample sites,including an uncultivated erosional site,an uncultivated depositional site,a Robinia pseudoacacia erosional site and a Robinia pseudoacacia depositional site in this study,taking the autotrophic bacteria with carbon-fixing functional gene cbb L in soil samples as the research object.High-throughput sequencing technology was used to study the diversity,distribution characteristics and structural differences of carbon-fixing bacterial communities.The results showed that the diversity and abundance of carbon-fixing bacteria on R.pseudoacacia slopes were significantly lower than those on uncultivated slopes(P<0.01).Sequencing data identified 21 autotrophic bacterial genera possessing cbb L carbon fixation genes belonging to 3 bacterial phyla,including Blastomonas,Proteobacteria and Actinomycetes.Principal coordinate analysis(PCoA)and cluster analysis showed that the autotrophic carbon-fixing bacterial communities were similar in eroded sites and sedimentary sites under the same slope.Correlation analysis shows that soil total potassium,total nitrogen,and pH have a high correlation with changes in bacterial community composition.

关 键 词：自养细菌 碳循环 黄土高原 高通量测序 土壤侵蚀 

分 类 号：S154.3[农业科学—土壤学]