Studies on Bioflocculant Production by Pseudoalteromonas sp. NUM8, a Marine Bacteria Isolated from the Circulating Seawater  

作　　者：FU Wandong LIAO Miaofei ZHANG Dongxu ZHOU Yufang YANG Huicheng 

机构地区：[1]Zhejiang Marine Development Research Institute,Zhoushan 316021,China [2]Marine Fishery Institute of Zhejiang Province,Key Laboratory of Mariculture&Enhancement of Zhejiang Province,Zhoushan 316021,China

出　　处：《Journal of Ocean University of China》2021年第5期1276-1284,共9页中国海洋大学学报（英文版）

基　　金：This work was supported by the Public Welfare Pro-jects in Zhejiang Province(No.LGN21C200001);the Public Welfare Projects in Zhoushan city(Nos.2021C 41005 and 2021C41007);the Natural Science Foun-dation of Marine Fishery Institute of Zhejiang Province(No.2020KF 010).

摘　　要：A bioflocculant producing potential bacteria was isolated from the circulating seawater of bio-filter using streak plate methods.The bacteria was identified through biochemical characteristics,partial 16S ribosomal ribonucleic acids(rRNA),nucleo-tide sequencing,and BLAST analysis of the gene sequence that showed the bacteria have 99%similarity to Pseudoalteromonas sp.and deposited in GenBank as Pseudoalteromonas sp.NUM8 with accession number JX435820.Influences of time course assay,carbon sources,nitrogen sources,inoculum size,as well as initial pH on the bacteria producing extracellular bioflocculant activity were investigated.The results showed that the strain optimal production period of microbial bioflocculant was at 72 h(flocculating activity of 94.5%),then dropped slowly.The bacteria optimally produced the bioflocculant when 1.0%sucrose and 0.5%sodium nitrate were used as sole sources of carbon and nitrogen with flocculating activities of 92.8%and 93.8%respectively.Also,the bacteria produced the bioflocculant optimally when initial pH of the medium was 5.0(flocculating activity 93.2%),and when Ca^(2+)was used as cation(flocculating activity 93.4%).The culture condition of inoculum size of 3%(v/v)was optimal flocculant pro-duction(flocculating activity 94.4%).Composition analyses indicated the bioflocculant to be principally a glycoprotein made up of about 34.3%protein and 63.4%total carbohydrate.

关 键 词：Pseudomonas sp.NUM8 BIOFLOCCULANT flocculating activity circulating seawater 

分 类 号：X172[环境科学与工程—环境科学]