Fucoidans from Thelenota ananas with 182.4 kDa Exhibited Optimal Anti-Adipogenic Activities by Modulating the Wnt/β-Catenin Pathway  

作　　者：LIU Yuanyuan WANG Na TIAN Yingying CHANG Yaoguang WANG Jingfeng 

机构地区：[1]College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China [2]Marine Biomedical Research Institute of Qingdao,Qingdao 266061,China

出　　处：《Journal of Ocean University of China》2021年第4期921-930,共10页中国海洋大学学报（英文版）

基　　金：This study was supported by the National Key Research and Development Program of China(No.2018YFC0311203);the Key Research and Development Plan in Shandong Province(No.2016YYSP017).

摘　　要：In this study,fucoidans were extracted from the sea cucumber Thelenota ananas(Ta-FUCs)by enzymatic degradation.Four products with molecular weights of 1380.3,524.0,182.4,and 110.3 kDa were obtained,and the Ta-FUC showing optimal anti-adipogenic activities was determined.Results of MTT and Oil red O staining analyses showed that the Ta-FUCs inhibited the proliferation and differentiation of 3T3-L1 adipocytes.Futhermore,Ta-FUCs significantly downregulated the key transcriptional factors,such as SREBP-1c,PPARγ,and C/EBPαof adipocytes.The Ta-FUCs also activated Wnt/β-catenin pathway-related genes,such asβ-catenin,LRP5,and FrZ.The Ta-FUCs suppressed lipid accumulation in 3T3-L1 adipocytes possibly by decreasing the expression of genes ACC,FAS,ME,GPAT,DGAT,and PILN,which are important in the synthesis of fatty acids and triglycerides;and by increasing the expression of genes PPARα,CPT-1α,and ACOX,which are crucial in fatty acidβ-oxidation.The anti-adipogenic activities initially increased and then declined with decreasing molecular weight.Among the Ta-FUCs,the 182.4 kDa Ta-FUC exhibited optimal bioactivities.This study reports for the first time that Ta-FUCs can prevent obesity by regulating the differentiation and lipid accumulation of adipocytes.

关 键 词：fucoidan from Thelenota ananas molecular weight anti-adipogenic activities adipocyte differentiation lipid accumulation Wnt/β-catenin pathway 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]