ZmbZIP76通过减轻活性氧损伤和渗透胁迫增强植物对盐碱胁迫的耐受性  

ZmbZIP76 Enhances Plant Tolerance to Saline-alkali Stress by Reducing ROS Damage and Osmotic Stress

作　　者：贺琳[1] 张苗苗 吴紫璇 孙立强 杨克军[1] He Lin;Zhang Miaomiao;Wu Zixuan;Sun Liqiang;Yang Kejun(Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学,大庆163319

出　　处：《黑龙江八一农垦大学学报》2021年第4期7-13,39,共8页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省青年创新人才(UNPYSCT-2018079);黑龙江省博士后资助经费(LBH-Z16165);黑龙江八一农垦大学青年创新人才(CXRC2016-03);黑龙江八一农垦大学学成引进人才科研启动计划(XYB2015-02)。

摘　　要：盐碱土在世界范围内广泛分布,尤其是耕地的次生盐渍化严重影响粮食作物的高产稳产。虽然许多bZIP转录因子在响应多种非生物胁迫中发挥重要作用,但它们在抗盐碱胁迫中的作用模式在很大程度上尚不清楚。研究利用实时定量PCR研究ZmbZIP76在盐碱胁迫和ABA处理下的表达模式,利用ZmbZIP76基因在拟南芥中的异源表达来鉴定ZmbZIP76蛋白的功能。表达模式分析显示ZmbZIP76可以被ABA和NaHCO_(3)显著诱导表达。NaHCO_(3)处理后,ZmbZIP76转基因拟南芥的根长是野生型根长的1.4~1.45倍ZmbZIP76转基因植株表现出显著提高的脯氨酸水平和抗氧化酶活性,显著降低的H_(2)O_(2)含量和丙二醛(MDA)含量。表明ZmbZIP76通过降低活性氧损伤和渗透胁迫增强盐碱耐受性,这将为进一步研究ZmbZIP76基因参与的植物盐碱胁迫反应的分子机制提供线索。Saline-alkali soil is widely distributed all over the world,especially the secondary salinization of cultivated land seriously affects the high and stable yield of grain crops.Although many bZIP transcription factors play important roles in response to multiple abiotic stresses,such as salt,osmotic and low temperature stresses,their mode of action in saline-alkaline stress resistance is largely unknown.In this study,the expression pattern of ZmbZIP76 under saline-alkali stress and ABA treatment was studied by real-time quantitative PCR,and the function of ZmbZIP76 protein was identified by heterologous expression of ZmbZIP76 gene in Arabidopsis thaliana.The expression pattern analysis showed that the expression of ZmbZIP76 was induced significantly by ABA and NaHCO_(3).After NaHCO_(3) treatment,the root length of ZmbZIP76 transgenic Arabidopsis was 1.4-1.45 times that of wild-type plants.In addition,ZmbZIP76 transgenic plants showed significantly increased proline levels and autioxidant enzyme activities,and decreased H_(2)O_(2) content and malondialdehyde(MDA)content under saline-alkaline stress.Together,these results suggest that ZmbZIP76 enhances the tolerance to saline-alkaline stress by reducing ROS damage and osmotic stress,which can provide clues for further study of the molecular mechanism of ZmbZIP76 gene involved in regulating plant saline-alkali stress response.

关 键 词：玉米 BZIP 拟南芥 ROS 盐碱胁迫 

分 类 号：Q78[生物学—分子生物学]