壳聚糖型SO_(2)缓释材料的制备及性质表征  

Preparation and Characterization of Chitosan-type SO_(2) Sustained-release Material

作　　者：杨晓君 周静 谢元 王秋平 丁泽人 赵立艳[3] 金鹏[3] YANG Xiao-jun;ZHOU Jing;XIE Yuan;WANG Qiu-ping;DING Ze-ren;ZHAO Li-yan;JIN Peng(College of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China;Xinjiang Key Laboratory of Postharvest Science and Technology,Urumqi 830052,China;College of Food Science and Technology,Nanjing Agriculture University,Nanjing 210000,China)

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052 [2]新疆果品采后科学与技术重点实验室,新疆乌鲁木齐830052 [3]南京农业大学食品科学技术学院,江苏南京210000

出　　处：《现代食品科技》2021年第8期176-183,共8页Modern Food Science and Technology

基　　金：中央高校基本科研业务费专项资金项目(KYYJ201707)。

摘　　要：采用金属离子配位反应、硫化反应和包埋技术,制备壳聚糖型SO_(2)缓释材料(Chitosan type SO_(2) sustained release material,CS-SO_(2)SM),以期从分子结构着手研发出SO_(2)新型葡萄保鲜剂。通过单因素实验,筛选最佳金属离子和硫化剂,结合析因实验设计,采用碘量法测定SO_(2)释放量和抑菌圈试验测定其抑菌性;并以SO_(2)释放曲线、释放天数为主要考察指标,抑菌性为辅助指标,优化CS-SO_(2)SM的制备工艺;通过场发射环境扫描电子显微镜-能谱仪对其形态进行观察,傅里叶红外光谱仪考察其官能团的变化。最佳工艺为选择Cu^(2+),四硫化双五亚甲基秋兰姆(TRA)0.5 g,焦亚硫酸钠0.5 g,搅拌1.5 h,制备得到的CS-SO_(2)SM为黑褐色粉末,电镜下为絮云状,主要官能团-OH、-NH、-CONH的特征吸收峰与壳聚糖相比均出现蓝移,SO_(2)释放曲线呈先上升后缓慢下降。该条件下制备得到的材料抑菌圈平均直径为19.25 mm,具有最佳抑菌效果。研究表明通过配位反应、硫化反应及包埋技术制备而成的CS-SO_(2)SM有望成为具有一定应用前景的SO_(2)新型保鲜剂。In this study,chitosan-type SO_(2) sustained-release material(CS-SO_(2)SM)was prepared by metal ion complexation reaction,vulcanization reaction and embedding technology,in order to develop a new SO_(2) preservative for grapes based on molecular structure.Through single factor experiments,the best metal ion(s)and vulcanizing agent(s)were screened.In combination with the factorial experiment design,the amount of released SO_(2) and the antibacterial activity were determined by the iodometric method and inhibition zone test,respectively.The preparation process of CS-SO_(2)SM was optimized through taking the SO_(2) release curve and release days as the main examination indices.Its morphology was observed by the field emission environmental scanning electron microscope-energy spectrometer,and the changes of its functional groups were examined by a Fourier infrared spectrometer.The optimal process conditions:Cu^(2+)as the metal ion,0.5 g of dipentamethylenethiuram tetrasulfide(TRA),0.5 g of sodium pyrosulfite,and preparation duration(with stirring)as 1.5 h.The prepared CS-SO_(2)SM was a dark brown powder which appeared as flocculated clouds under the electron microscope.The characteristic absorption peaks of the main functional groups,-OH,-NH and-CONH showed blue shifts compared with chitosan.The SO_(2) release curve showed a rise first and then a slow decline.The materials prepared under these conditions had an average diameter of 19.25 mm for the inhibition zone and exhibited the strongest antibacterial effect.The research has shown that CS-SO_(2)SM prepared by coordination reaction,vulcanization reaction and embedding technology is expected to be a new type of SO_(2) preservative with a certain application prospect.

关 键 词：壳聚糖 配位反应 析因实验 二氧化硫缓释材料 

分 类 号：TS201.2[轻工技术与工程—食品科学]