四氯化碳致大鼠肝纤维化动物模型的文献再评价  

Re-evaluation of Literature on Rat Liver Fibrosis Animal Model Induced by Carbon Tetrachloride

作　　者：李高辉 吕文良[1] 闫暾 李娟梅[1] 武庆娟 赵婷[1] LI Gaohui;LV Wenliang;YAN Tun;LI Juanmei;WU Qingjuan;ZHAO Ting(Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]中国中医科学院广安门医院感染疾病科,北京100053 [2]北京中医药大学,北京100029

出　　处：《实验动物科学》2021年第3期39-46,共8页Laboratory Animal Science

基　　金：国家自然科学基金面上项目(No.81774282);国家重点研发计划“中医药现代化研究”重点专项(2018YFC1705700)。

摘　　要：目的通过相关文献阅读分析,比较目前利用四氯化碳(CCl4)诱导肝纤维化的造模方法,制订出最佳的造模方案,为科学研究提供参考借鉴。方法计算机检索数据库(中国知网、万方数据知识服务平台、维普网)获取近五年相关文献,选取实验病理结果和血清学检查谷丙转氨酶(ALT)、谷草转氨酶(AST)对文献进行评分。对相关实验影响因素做出评价,进而制定出模型诱导的标准。结果模型组病理结果均显示出正常肝小叶结构被破坏和小叶间纤维间隔形成,血清检查ALT和AST指标较空白对照组均有明显的增长。结论四氯化碳油剂诱导肝纤维化模型是可靠的实验方案,且动物品系、药物作用途径、四氯化碳浓度等因素均对实验结果产生影响。To compare the current method of liver fibrosis in rats induced by tetrachloromethane,and to establish the best method of liver fibrosis in order to provide reference for scientific research.Method Cnki,Wanfang data and cqvip were used to search and obtain the related literatures,data and the experimental pathological results and serological examination were selected,the literature was graded according to pathology and Alanine transaminase and aspartate transaminase rates.The relevant experimental factors are evaluated,and then the criteria of model induction are established.Result In the model group,the normal hepatic lobule structure was destroyed and the interlobular fibrous septum was formed.The serum ALT and AST indexes were significantly increased.Conclusion The reliable experimental scheme of hepatic fibrosis model induced by tetrachloromethane oil is reliable.species,sex,pathway of drug action and concentration of CCl_(4) all influence the experimental result.

关 键 词：四氯化碳 肝纤维化 动物模型 大鼠 文献评价 

分 类 号：R-332[医药卫生]