无花果叶片多糖抑制胃癌细胞增殖与促进凋亡效应  

Inhibition of proliferation and promotion of apoptosis of gastric cancer cells by leaf polysaccharides of Ficus carica

作　　者：邓佳丽 李晓[1] 安玉艳[1] 林泽崑 汪良驹[1,2] DENG Jia-li;LI Xiao;AN Yu-yan;LIN Ze-kun;WANG Liang-ju(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Qingdao Haidekun Institute of Biological Sciences,Qingdao 266200,China)

机构地区：[1]南京农业大学园艺学院,南京210095 [2]青岛海德坤生物科学研究院,青岛266200

出　　处：《天然产物研究与开发》2021年第8期1282-1291,共10页Natural Product Research and Development

基　　金：国家自然科学基金(31772253)。

摘　　要：为了明确无花果叶片多糖抑癌效应,本文以‘布兰瑞克’、‘玛斯义陶芬’和‘波姬红’三个无花果品种为材料,分别从7月至11月份叶片中提取多糖,并且研究其对人体胃癌SGC-7901细胞增殖的抑制效应,发现‘布兰瑞克’9月份叶片多糖抑制效应最高,2 mg/mL的抑制率达46.67%。以该时期叶片多糖为材料,研究发现,不同浓度多糖对癌细胞周期具有明显的阻滞作用。经多糖处理的癌细胞,活性氧含量和细胞凋亡率上升,促细胞凋亡基因Bax和p53表达量在mRNA水平上升,抗凋亡基因Bcl-2以及细胞周期相关基因,包括Cyclins和CDKs等表达量下降。以上结果表明,无花果叶片多糖可以诱导人体胃癌细胞SGC-7901细胞凋亡基因表达,抗凋亡基因和周期基因表达受抑,细胞活性氧上升,抑制细胞增殖,促进细胞凋亡。本研究结果为无花果叶片多糖运用于抗肿瘤药物开发提供了理论依据。In order to elucidate the anti-cancer effects of polysaccharides in fig(Ficus carica L.)leaves,three fig varieties,‘Brunswick’,‘Masui Dauphine’and‘Bo Jihong’were used in the experiments.The polysaccharides were extracted from fig leaves which were harvested from July to November and their inhibitory effects on human gastric cancer SGC-7901 cells were investigated.It was found that all examples exhibited a certain inhibitory effect on SGC-7901 cells,among which,the leaf polysaccharide of‘Brunswick’in September was the most effective,with an inhibition of 46.67%at a dosage of 2 mg/mL.Using the leaf polysaccharides of‘Brunswick’in this period for the following experiments,it was found that the cycle of cancer cells was significantly blocked by different concentrations of polysaccharides.The content of ROS and the rate of apoptosis were significantly promoted after polysaccharide treatments.The expressions of pro-apoptotic genes Bax and p53 were up-regulated at the mRNA level,while that of anti-apoptotic genes Bcl-2 and cell cycle-related genes,including Cyclins and CDKs,were down-regulated.The above results indicate that the polysaccharides from fig leaves can increase the apoptotic gene expressions of human gastric cancer SGC-7901 cells,depress that of anti-apoptotic genes,stimulate cellular ROS burst,block cell proliferation,and stimulate cell apoptosis.These findings provide theoretical basis for utilization of fig leaf polysaccharides for anti-cancer medicine.

关 键 词：无花果叶 多糖 胃癌 细胞凋亡 基因表达 

分 类 号：R735.2[医药卫生—肿瘤]