“Candidatus anaplasma camelii”in onehumped camels(Camelus dromedarius)in Morocco:a novel and emerging Anaplasma species?  

作　　者：Hicham Ait Lbacha Zaid Zouagui Said Alali Abdelkbir Rhalem Elisabeth Petit Marie Julie Ducrotoy Henri-Jean Boulouis Renaud Maillard 

机构地区：[1]Institut Agronomique et Veterinaire Hassan II,Rabat,Morocco [2]Departement de medecine,chirurgie et reproduction,Madinat Al Irfane,Rabat Instituts,IAV Hassan II,BP 6202,Rabat 10101,Morocco [3]Anses,ENVA,INRA,BIPAR,Maisons-Alfort,France [4]Division of Infection and Pathway Medicine,School of Biomedical Sciences,College of Medicine and Veterinary Medicine,The University of Edinburgh,Edinburgh,UK [5]Ceva Sante Animale,Libourne,France [6]INP-ENVT,Toulouse,France

出　　处：《Infectious Diseases of Poverty》2017年第1期1-8,共8页贫困所致传染病（英文）

基　　金：This work was supported by the PRAD project under grant agreement number 28027YM;the Institut Agronomique et Veterinaire Hassan II,BIPAR and Ecole Nationale Veterinaire de Toulouse。

摘　　要：Background:There has been a growing interest in camel anaplasmosis due to its recent emergence in this reservoir species and concerns for its zoonotic potential.The epidemiology of anaplasmosis in camels therefore remains poorly understood mostly because camels belong to marginalised poor and often transhumant populations whose interests are largely neglected.Most studies of anaplasmosis in camels have relied on microscopy and serology for diagnosis and only three studies,undertaken in Tunisia,Saudia Arabia and China,have used molecular diagnostics.The present work characterises Anaplasmataceae strains circulating in the Camelus dromedarius reservoir in Morocco using PCR.Methods:Camels(n=106)were randomly sampled from 6 regions representing different agro-ecological areas in southern Morocco.Whole blood was collected and screened using PCR methods targeting the gene groEL.Anaplasmataceae strains were characterised by sequence analysis of the gene groEL.Results:A total of 39.62%(42/106)camels screened were positive for Anaplasmataceae spp.GenBank BLAST analysis of five positive sequenced samples revealed that all strains were 100%identical to“Candidatus Anaplasma camelii”.Phylogenetic investigation and genetic characterisation of the aligned segment(650 bp)of the gene groEL confirmed high similarity with A.platys.Conclusion:This study demonstrates the circulation of a previously unidentified species of the genus Anaplasma in Morocco which is genetically close to the agent causing canine anaplasmosis but whose main reservoir is thought to be Camelus dromedarius.Trial registration number:This study is not a clinical trial and therefore a trial registration number does not apply.

关 键 词：Anaplasma camelii TICK-BORNE One-humped camel(Camelus dromedarius) GROEL PHYLOGENY Morocco 

分 类 号：R73[医药卫生—肿瘤]