Levels of insecticide resistance to deltamethrin,malathion,and temephos,and associated mechanisms in Aedes aegypti mosquitoes from the Guadeloupe and Saint Martin islands(French West Indies)  

作　　者：Daniella Goindin Christelle Delannay Andric Gelasse Cedric Ramdini Thierry Gaude Frederic Faucon Jean-Philippe David Joël Gustave Anubis Vega-Rua Florence Fouque 

机构地区：[1]Laboratory of Medical Entomology,Unit Environment and Health,Pasteur Institute of Guadeloupe,97183 Les Abymes,Guadeloupe [2]Vector control Service,Regional Health Agency,Dothemare,Les Abymes,Guadeloupe [3]Alpine Ecology Laboratory(LECA),CNRS,UMR 5553,2233 rue de la piscine BP53,38041 Grenoble,Cedex 9,France [4]University of Grenoble Alpes,Grenoble,France [5]Environmental and Systems Biology(BEeSy),University of Grenoble Alpes,Grenoble,France [6]Vector Environment and Society Unit,Special Programme for Research and Training in Tropical Diseases(TDR),World Health Organization,20,Avenue Appia,CH-1211 Geneva 27,Switzerland

出　　处：《Infectious Diseases of Poverty》2017年第1期339-353,共15页贫困所致传染病（英文）

基　　金：This study was financially supported by the Fonds Europeen de Developpement Economique et Regional project FED-1-1.432932;by the ARS through the collaboration convention no°2014-140116;DG was supported by La Region Guadeloupe,Le Fond Social Europeen,and the University of Antilles。

摘　　要：Background:In the Guadeloupe and Saint Martin islands,Aedes aegypti mosquitoes are the only recognized vectors of dengue,chikungunya,and Zika viruses.For around 40 years,malathion was used as a mosquito adulticide and temephos as a larvicide.Since the European Union banned the use of these two insecticide molecules in the first decade of the 21st century,deltamethrin and Bacillus thuringiensis var.israelensis are the remaining adulticide and larvicide,respectively,used in Guadeloupe.In order to improve the management of vector control activities in Guadeloupe and Saint Martin,we investigated Ae.aegypti resistance to and mechanisms associated with deltamethrin,malathion,and temephos.Methods:Ae.aegypti mosquitoes were collected from six different localities of Guadeloupe and Saint Martin.Larvae were used for malathion and temephos bioassays,and adult mosquitoes for deltamethrin bioassays,following World Health Organization recommendations.Knockdown resistance(Kdr)genotyping for V1016I and F1534C mutations,and expression levels of eight enzymes involved in detoxification mechanisms were examined in comparison with the susceptible reference Bora Bora strain.Results:Resistance ratios(RR50)calculated for Ae.aegypti larvae showed high resistance levels to temephos(from 8.9 to 33.1-fold)and low resistance levels to malathion(from 1.7 to 4.4-fold).Adult females displayed moderate resistance levels to deltamethrin regarding the time necessary to affect 50%of individuals,varying from 8.0 to 28.1-fold.Molecular investigations on adult mosquitoes showed high resistant allele frequencies for V1016I and F1534C(from 85 to 96%and from 90 to 98%,respectively),as well as an overexpression of the glutathione S-transferase gene,GSTe2,the carboxylesterase CCEae3a,and the cytochrome genes 014614,CYP6BB2,CYP6M11,and CYP9J23.Conclusions:Ae.aegypti populations from Guadeloupe and Saint Martin exhibit multiple resistance to organophosphates(temephos and malathion),and pyrethroids(deltamethrin).The mechanisms associated with these resistanc

关 键 词：Aedes aegypti MOSQUITOES Insecticide resistance DELTAMETHRIN MALATHION Temephos GUADELOUPE Saint Martin 

分 类 号：R73[医药卫生—肿瘤]