New insights on serodiagnosis of trichinellosis during window period:early diagnostic antigens from Trichinella spiralis intestinal worms  

作　　者：Zhong-Quan Wang Ya-Li Shi Rou-Dan Liu Peng Jiang Ya-Yi Guan Ying-Dan Chen Jing Cui 

机构地区：[1]Department of Parasitology,Medical College,Zhengzhou University,Zhengzhou 450052,China [2]National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Key Laboratory of Parasite and Vector Biology,Ministry of Health,Shanghai 200025,China

出　　处：《Infectious Diseases of Poverty》2017年第1期368-371,共4页贫困所致传染病（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(no.81572024 and 81672043);The funders had no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.

摘　　要：The clinical diagnosis of trichinellosis is difficult because its clinical manifestations are nonspecific.Detection of anti-Trichinella IgG by ELISA using T.spiralis muscle larval excretory-secretory(ES)antigens is the most commonly used serological method for diagnosis of trichinellosis,but the main disadvantage is false negativity during the early stage of infection.There is an obvious window period between Trichinella infection and antibody positivity.During the intestinal stage of Trichinella infection,the ES antigens of intestinal worms(intestinal infective larvae and adults)are exposed to host’s immune system at the earliest time and elicit the production of specific anti-Trichinella antibodies.Anti-Trichinella IgG antibodies in infected mice were detectable by ELISA with ES antigens of intestinal worms as soon as 8-10 days post infection(dpi),but ELISA with muscle larval ES antigens did not permit detection of infected mice before 12 dpi.Therefore,the new early antigens from T.spiralis intestinal worms should be screened,identified and characterized for early serodiagnosis of trichinellosis.

关 键 词：TRICHINELLOSIS Early serodiagnosis Intestinal infective larvae Adult worms 

分 类 号：R57[医药卫生—消化系统]