Identification and characterization of the causative triatomine bugs of anaphylactic shock in Zhanjiang,China  

作　　者：Ya-Lan Huang Da-Na Huang Wei-Hua Wu Fan Yang Xiao-Min Zhang Miao Wang Yi-Jun Tang Qian Zhang Li-Fei Peng Ren-Li Zhang 

机构地区：[1]Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China [2]Department of Parasitology,Guangdong Medical University,Zhanjiang 524005,China

出　　处：《Infectious Diseases of Poverty》2018年第1期1325-1333,共9页贫困所致传染病（英文）

基　　金：This work was supported by National Key Research and Development Program of China(No.2016YFC1202000,2016YFC1202001,and 2016YFC1200500);Shenzhen San-Ming Project for prevention and research on vector-borne diseases(SZSM201611064).

摘　　要：Background:Two health concerns primarily related to triatomine bugs are transmission of Trypanosoma cruzi through infective feces,and allergic reactions induced by triatomine bites.In the Southwestern United States,reduviid bugs bites commonly cause insect allergy.In South China,four cases of anaphylactic shock have been reported after this bite exposure.To further classify the species of these bugs and confirm the sensitization of the triatomine saliva,we caught triatomine bugs from the region where the bites occurred and performed phylogenetic and immunohistochemical(IHC)analysis.Methods:Triatomine bugs were collected in Donghai Island of Zhanjiang City in South China.The genomic DNA was extracted from three legs of the bugs.The fragments of mitochondrial 16S rRNA,cytochrome c oxidase subunit I(COI)gene and nuclear ribosomal 18S and 28S rRNA genes were obtained by PCR and sequenced.A phylogenetic tree was constructed based on the sequence of 16S rRNA gene using a maximum likelihood method with MEGA 7.0 software.Trypanosomal specific fragments and vertebrate COI genes were amplified from the fecal DNA to detect the infection of trypanosomes and analyze the blood feeding patterns,respectively.Paraffin-embedded sections were then prepared from adult triatomines and sent for IHC staining.Results:We collected two adult triatomine bugs in Donghai Island.Morphological and molecular analyses indicated that the triatomines were Triatoma rubrofasciata.No fragments of T.cruzi or other trypanosomes were detected from the fecal DNA.Mitochondrial gene segments of Homo sapiens and Mus musculus were successfully amplified.The allergens which induced specific IgE antibodies in human serum were localized in the triatomine saliva by IHC assay.Conclusions:The two triatomine bugs from Donghai Island were T.rubrofasciata.They had bitten humans and mice.Their saliva should contain the allergens related to the allergic symptoms and even anaphylactic shock of exposed residents.Great consideration should be given to this triatomine bugs

关 键 词：Triatoma rubrofasciata Chagas disease Trypanosoma cruzi Anaphylactic shock 

分 类 号：R73[医药卫生—肿瘤]