生物条形码检测技术及其研究进展  

Detection technology and research progress of biological barcode

作　　者：刘培 尹会敏 穆文静 栗慧 武二斌[4] 魏东 LIU Pei;YIN Huimin;MU Wenjing;LI Hui;WU Erbin;WEI Dong(Hebei Key Laboratory of Quality&Safety Analysis-Testing for Agro-Products and Food,Hebei North University,Zhangjiakou,Hebei 075000,China;College of Agriculture and Forestry Science and Technology of Hebei North University,Zhangjiakou,Hebei 075000,China;Key Laboratory of Quality and Safety of Characteristic Agricultural Products of Zhangjiakou,Hebei North University,Zhangjiakou,Hebei 075000,China;Zhangjiakou City Animal Health Supervision Institute,Zhangjiakou,Hebei 075000,China)

机构地区：[1]河北北方学院河北省农产品食品质量安全分析检测重点实验室,河北张家口075000 [2]河北北方学院农林科技学院,河北张家口075000 [3]河北北方学院张家口市特色农产品质量安全重点实验室,河北张家口075000 [4]张家口市动物卫生监督所,河北张家口075000

出　　处：《生物安全学报》2021年第3期172-177,共6页Journal of biosafety

基　　金：河北省重点研发计划项目(19225504D);河北省高校百名优秀创新人才支持计划(Ⅲ)(SLRC2017019);河北北方学院博士基金(12995545);2021年河北省研究生创新资助项目(CXZZSS2021125)。

摘　　要：生物条形码检测技术作为一种新的诊断工具,已逐渐应用于蛋白质、核酸和小分子物质的检测。生物条形码技术通过构建“纳米金颗粒-目标物-磁纳米颗粒”三明治结构,利用磁场作用,将结合在纳米金颗粒表面的大量相同序列的寡聚核苷酸洗脱下来,并进一步放大信号,实现对目标物的间接或直接检测。本文介绍了生物条形码检测技术的原理及其应用,综述了生物条形码与生物芯片银染技术、酶标纳米金技术、生物传感器技术、微孔板银染技术以及聚合酶链式反应技术等高灵敏放大信号技术的联合应用;并对其前景进行了展望,探索其最佳反应条件、优化操作步骤和多种物质的残留检测,开发标准化、商业化的生物条形码检测技术免疫试剂盒是未来重要的研究方向。Bio-barcode technology,as a new diagnostic tool,has been gradually applied to the detection of proteins,nucleic acids,and small-molecule compounds.Based on the sandwich structure of gold nanoparticles and using magnetic field effects,the bio-barcode technology aims to amplify the signal after eluting numerous oligonucleotides of the same sequence on the surface of gold nanoparticles and complete the detection of the target directly and indirectly.In this study,the principle and application of bio-barcodes are introduced,and the joint application of bio-barcode and biochip silver-dyeing technology,enzyme-labeled gold nanoscale technology,biosensor technology,microplate silver-dyeing technology,and polymerase chain reaction technology are reviewed.Considering the future outlook of this technology,it is of great significance to explore the best reaction conditions,optimize the operation procedures,explore the residue detection of a variety of substances,and develop standardized and commercial immunity kits for bio-barcode technology in the future.

关 键 词：生物条形码检测技术 蛋白质 核酸 小分子物质 三明治结构 纳米金颗粒 

分 类 号：Q-1[生物学]