Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters  

作　　者：Amanda P.N.Quintal Bruna C.Borges Paula C.Brígido Rebecca T.Silva Ana F.Notário Marlus A.Santos Maria A.de Souza Fernanda G.O.Nascimento Antônio V.Mundim Guilherme M.J.Costa André B.Vasconcelos Claudio V.da Silva 

机构地区：[1]Instituto de Ciências Biomédicas,Universidade Federal de Uberlândia,Uberlândia,Minas Gerais,Brasil [2]Hospital Veterinário,Universidade Federal de Uberlândia,Uberlândia,Minas Gerais,Brasil [3]Departamento de Ciências Biomédicas,Universidade Federal de Minas Gerais,Belo Horizonte,Brasil [4]Universidade de Uberaba,Uberaba,Minas Gerais,Brasil [5]Departamento de Imunologia–Instituto de Ciências Biomédicas,Universidade Federal de Uberlândia,Campus Umuarama,Uberlândia,MG,Brasil

出　　处：《Infectious Diseases of Poverty》2016年第1期261-265,共5页贫困所致传染病（英文）

基　　金：supported by grants and fellowships from Fundação de AmparoàPesquisa do Estado de Minas Gerais,Conselho Nacional de Desenvolvimento Científico e Tecnológico,and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.

摘　　要：Background:Leishmaniasis causes alterations and lesions in the genital system,which leads to azoospermia and testicular atrophy in animals during the chronic phase of the infection.The aim of this study was to reveal the kinetics of Leishmania chagasi infection in the genital system of male golden hamsters(Mesocricetus auratus).Methods:Animals were intraperitoneally inoculated with amastigotes from L.chagasi.At different time points animals were euthanized and genital organs processed for histo-pathological,qPCR,cytokines and testosterone detection assays.Results:Our results showed a high parasite load in testis,followed by an increase of pro-inflammatory cytokines IL1-β,TNF-αand IFN-γ,and testosterone.Subsequently,IL-4 expression was upregulated and basal parasite persistence in testis was observed using the experimental approach.Conclusion:Extracellular amastigotes migrated to the epididymis posing as a potential major factor of parasite persistence and venereal transmission of L.chagasi infection in hamsters.

关 键 词：Leishmania chagasi Extracellular amastigotes TESTIS EPIDIDYMIS Immune response TESTOSTERONE 

分 类 号：R53[医药卫生—内科学]