Low activity of complement in the cerebrospinal fluid of the patients with various prion diseases  

作　　者：Cao Chen Yan Lv Qi Shi Wei Zhou Kang Xiao Jing Sun Xiao-Dong Yang Xiao-Ping Dong 

机构地区：[1]State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Chang-Bai Rd 155,Beijing 102206,China [2]Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,Zhejiang University,Hangzhou 310003,China [3]Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Infectious Diseases of Poverty》2016年第1期300-306,共7页贫困所致传染病（英文）

基　　金：supported by Chinese National Natural Science Foundation Grants(81401670);China Mega-Project for Infectious Disease(2011ZX10004-101,2012ZX10004215);the SKLID Development Grant(2012SKLID102).

摘　　要：Background:The aim of this study was to analyze the state of activity and levels of complement in the cerebrospinal fluid(CSF)of patients with various prion diseases(PrDs).Findings:The proteomic data emphasized the levels of 20 known complement components found in the CSF of the sCJD panel that were lower than those found in the non-PrD panel.50%of the complement hemolytic activity(CH50)assays revealed significantly lower activity of complement in the CSF of the sCJD panel.The decreased levels of three key complement subunits,C3a/α,C4β,and C9 in the CSF of the sCJD panel were verified by Western blots.Furthermore,the CH50 values in the CSF of 136 sCJD,39 gCJD,22 FFI and 145 non-CJD patients were individually tested.Compared with the control of non-PrD,the CH50 value in the CSF specimens of various PrDs,especially in three subtypes of inherited PrDs,were significantly lower.Relationship analysis identified that the CH50 activity in the CSF was negatively associated with the protein 14–3–3 positive in the CSF.Conclusion:These results indicate a silent complement system in the CSF of PrD patients.

关 键 词：Prion diseases CSF Complement component CH50 sCJD 

分 类 号：R73[医药卫生—肿瘤]