维生素D对糖尿病大鼠模型主动脉中层厚度的影响及其作用机制  

The effect of Vit-D on the thickness of middle-layer of the aorta in the DM rat model and the mechanism of action of that

作　　者：关连颖[1] 李书瑞[2] 康凯宁[2] 张绍义[1] GUAN Lian-ying;LI Shu-rui;KANG Kai-ning(The First Department of Aged Disease,Handan Central Hospital,Handan 056002,China;不详)

机构地区：[1]邯郸市中心医院老年病一科,河北邯郸056002 [2]邯郸市中心医院家庭病床科,河北邯郸056002

出　　处：《中国医学装备》2021年第8期197-201,共5页China Medical Equipment

摘　　要：目的:探究维生素D(Vit-D)对糖尿病(DM)大鼠模型主动脉中层厚度的影响及其蛋白激酶受体样内质网激酶(PERK)和真核翻译起始因子2α(eIF2α)(PERK/eIF2α)通路的作用机制。方法:选用30只Sprague-Dawley(SD)雄性大鼠,采用随机数表法将其分为对照组、DM组和DM+Vit-D组,每组10只。通过给每只大鼠腹腔注射链脲佐菌素建立大鼠DM模型,DM+Vit-D组大鼠使用活性形式的Vit-D3灌胃(0.1μg·kg^(-1)·d^(-1))。比较各组大鼠的血糖水平、主动脉损伤情况、血清一氧化氮(NO)和内皮素(ET)水平,并采用Westernblot检测主动脉组织中PERK/eI F2α通路中的蛋白表达水平。结果:DM模型大鼠的血糖水平均>16.7 mmol·L^(-1),干预后DM组和DM+Vit-D组的尾静脉空腹血糖(FBG)水平差异无统计学意义。DM组的收缩压和脉搏波传导速度(PWV)显著高于对照组,其差异有统计学意义(t=5.263,t=4.956,P<0.05);DM+Vit-D组的收缩压和PWV显著低于DM组,其差异有统计学意义(t=5.016,t=4.332;P<0.05)。DM组大鼠的主动脉中层厚度[(103.24±4.98)μm]显著高于对照组[(90.15±3.24)μm],DM+Vit-D组的主动脉中层厚度[(96.74±4.21)μm]显著低于DM组[(80.25±2.86)μm],差异有统计学意义(t=3.521,t=3.852;P<0.05)。DM组的NO水平为(51.49±6.85)μmol·L^(-1)显著降低、ET水平为(110.28±11.39)pg·ml^(-1)显著升高,与对照组比较差异有统计学意义(t=4.585,t=5.677;P<0.05);DM+Vit-D组的NO水平[(61.18±8.21)μmol·L^(-1)]显著升高,ET水平[(101.44±11.32)pg·ml^(-1)]显著降低,与DM组比较差异有统计学意义(t=5.029,t=5.881;P<0.05)。DM组大鼠的PERK和eIF2α蛋白水平显著高于对照组,差异有统计学意义(t=3.612,t=4.083;P<0.05);DM+Vit-D组的PERK和eIF2α蛋白水平显著低于DM组,差异有统计学意义(t=3.506,t=3.892;P<0.05)。结论:Vit-D可通过抑制DM大鼠的主动脉中PERK/eIF2α通路水平,缓解主动脉损伤和血管内皮功能。Objective:To explore the effects of vitamin D(Vit-D)on the thickness of middle-layer of aorta in the diabetes mellitus(DM)rat and the mechanism of action of the pathway of protein kinase receptor like endoplasmic reticulum kinase(PERK)and eukaryotic translation initiation factor 2α(eIF2α)(PERK/eIF2α).Methods:Thirty Sprague Dawley(SD)male rats were randomly divided into control group,DM group and DM+Vit-D group,with 10 rats in each group.DM model was established by intraperitoneal injection of streptozotocin to each rat in DM group.Rats in DM+Vit-D group received gavage with active form of Vit-D3(0.1μg·kg^(-1)·d^(-1)).The levels of blood glucose,aortic injury,serum nitric oxide(NO)and endothelin(ET)of among different groups were compared,and Western blot was adopted to detect the expression levels of proteins in PERK/eIF2 pathway of aortic tissue.Results:The blood glucose level of all DM model rats was larger than 16.7 mmol·L^(-1).After intervention,there was no significant difference in the level of fasting blood glucose(FBG)of caudal vein between DM group and DM+Vit-D group.Systolic blood pressure and pulse wave velocity(PWV)in DM group were significantly higher than those in control group(t=5.263,t=4.956,P<0.05),respectively.Systolic blood pressure and PWV in DM+Vit-D group were significantly lower than those in DM group(t=5.016,t=4.332,P<0.05).The thickness of aortic middle layer of DM group[(103.24±4.98)μm]was significantly higher than that of control group[(90.15±3.24)μm].The thickness of aortic middle layer in DM+Vit-D group[(96.74±4.21)μm]was significantly lower than that in DM group[(80.25±2.86)μm],the differences of them between two groups were statistically significant(P<0.05).Compared with the control group,the level of NO[51.49±6.85]was significantly decreased and the level of ET[110.28±11.39]was increased significantly in DM group(t=4.585,t=5.677,P<0.05).NO level in DM+Vit-D group[(61.18±8.21)μmol·L^(-1)]was increased,and the ET level of that group[(101.44±11.32)PG·mol·L^(-1)]w

关 键 词：糖尿病 主动脉 维生素D(Vit-D) 蛋白激酶受体样内质网激酶(PERK) 真核翻译起始因子2α(eIF2α) 通路 

分 类 号：R-332[医药卫生]