PBMC中CARD9、MDC、IL⁃32在溃疡性结肠炎诊断和病情评估中价值  

The value of CARD9,MDC and IL⁃32 in PBMC in diagnosis and evaluation of ulcerative colitis

作　　者：谢玥[1] 张绍衡 熊瑶 于长辉[1] XIE Yue;ZHANG Shaoheng;XIONG Yao;YU Changhui(Department of Gastroenterology,Zhujiang Hospital,Southern Medical University,Guangzhou,Guang-dong,China,528200)

机构地区：[1]南方医科大学珠江医院消化内科,广东广州528200

出　　处：《分子诊断与治疗杂志》2021年第8期1329-1332,共4页Journal of Molecular Diagnostics and Therapy

基　　金：广东省自然科学基金项目(2018A030313817)。

摘　　要：目的探讨外周血单个核细胞(PBMC)中胱天蛋白酶募集域蛋白9(CARD9)、髓样树突状细胞(MDC)、白介素⁃32(IL⁃32)在溃疡性结肠炎(UC)诊断和病情评估中价值。方法选取2019年1月至2021年1月南方医科大学珠江医院消化内科收治的82例UC患者(UC组)及体检中心40例健康体检人群(对照组),比较两组CARD9 mRNA、MDC、IL⁃32 mRNA、CARD9蛋白、IL⁃32蛋白,采用受试者工作特征曲线(ROC)分析各指标诊断UC价值,比较UC患者不同病情程度者各检测指标水平,采用Spearman及多分类Logistic回归方程分析各指标与UC活动期患者病情程度关系。结果UC组CARD9 mRNA、MDC、IL⁃32 mRNA、CARD9蛋白、IL⁃32蛋白高于对照组,差异有统计学意义(P<0.05);CARD9 mRNA、MDC、IL⁃32 mRNA诊断UC的AUC依次为0.712、0.901、0.739,各指标联合的AUC为0.920;活动期患者CARD9 mRNA、MDC、IL⁃32 mRNA高于缓解期(P<0.05),随着活动期患者病情程度加重,CARD9 mRNA、MDC、IL⁃32 mRNA依次增高,两两比较差异均有统计学意义(P<0.05);Spearman相关性分析显示,CARD9 mRNA(r=0.808,P<0.001)、MDC(r=0.815,P<0.001)、IL⁃32 mRNA(r=0.874,P<0.001)、CARD9蛋白(r=0.735,P<0.001)、IL⁃32蛋白(r=0.803,P<0.001)均与病情程度呈正相关;多分类Logistic回归方程分析显示,CARD9 mRNA、MDC、I L⁃32 mRNA、CARD9蛋白、IL⁃32蛋白是UC患者病情程度的相关独立影响因素(P<0.05)。结论UC患者PBMC中CARD9 mRNA、MDC、IL⁃32 mRNA、CARD9蛋白、IL⁃32蛋白显著升高,并与病情程度有关,联合检测CARD9 mRNA、MDC、IL⁃32 mRNA能为临床诊断UC及评估UC病情提供参考。Objective To investigate the effect of caspase recruitment domain protein 9(CARD9),myeloid dendritic cells(MDC)and interleukin⁃32(IL⁃32)in peripheral blood mononuclear cells(PBMC)on ulcerative colitis(UC)and the value of diagnosis and disease assessment.Methods From Janu⁃ary 2019 to January 2021,82 UC patients(UC group)and 40 healthy people in the physical examination cen⁃ter(control group)were selected to compare CARD9 mRNA,MDC,IL⁃32 mRNA,CARD9 Protein,IL⁃32 protein between the two groups.The receiver operating characteristic curve(ROC)used to analyze the diagnos⁃tic value of each indicator,and the level of each detection indicator in UC patients with different levels of dis⁃ease was compared.Spearman and multi⁃class logistic regression equation were used to analyze the relationship between each indicator and the patient's condition in the active UC phase.Results CARD9 mRNA,MDC,IL⁃32 mRNA,CARD9 protein,and IL⁃32 protein in the UC group were higher than those in the control group,the difference was statistically significant(P<0.05).The AUC of CARD9 mRNA,MDC,IL⁃32 mRNA for diagnosis of UC was 0.712,0.901,0.739,and the AUC of the combination of each indicator was 0.920.CARD9 mRNA,MDC,and IL⁃32 mRNA in active patients were higher than those in remission(P<0.05).With the se⁃verity of disease in active patients,CARD9 mRNA and MDC,IL⁃32 mRNA increased,and the difference be⁃tween pairwise comparisons was statistically significant(P<0.05).Spearman correlation analysis showed that CARD9 mRNA(r=0.808,P<0.001),MDC(r=0.815,P<0.001),IL⁃32 mRNA(r=0.874,P<0.001),CARD9 protein(r=0.735,P<0.001),IL⁃32 protein(r=0.803,P<0.001)are positively correlated with the se⁃verity of the disease.Multi⁃class logistic regression analysis showed that CARD9 mRNA,MDC,IL⁃32 mRNA,CARD9 protein,and IL⁃32 protein were independent factors affecting the severity of UC patients(P<0.05).Conclusion CARD9 mRNA,MDC,IL⁃32 mRNA,CARD9 protein,and IL⁃32 protein in PBMC of UC pa⁃tients are significantly increased

关 键 词：PBMC CARD9 MDC IL⁃32 溃疡性结肠炎 病情评估 

分 类 号：R574.62[医药卫生—消化系统]