高效液相色谱法测定化妆品中L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8的含量  

A method for examination of L-carnosine,eyeseryl,glutathione and acetyl hexapeptide-8 in cosmetics by HPLC

作　　者：许勇[1,2] 韩晶 潘晨[1,2] 彭兴盛 郑荣[1,2] XU Yong;HAN Jing;PAN Chen;PENG Xing-sheng;ZHENG Rong(Shanghai Institute for Food and Drug Control,Shanghai 201203,China;NMPA Key Laboratory for Monitoring and Evaluation of Cosmetics,Shanghai 201203,China)

机构地区：[1]上海市食品药品检验所,上海201203 [2]国家药品监督管理局化妆品监测评价重点实验室,上海201203

出　　处：《上海预防医学》2021年第8期745-749,共5页Shanghai Journal of Preventive Medicine

摘　　要：【目的】建立高效液相色谱法测定化妆品中L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8的测定方法。【方法】样品经水提取,以Agilent Eclipse XDB-C18(5μm,4.6 mm×150 mm)液相色谱柱进行分离,以甲醇和含有0.1%甲酸(体积分数,V/V)水溶液梯度洗脱,流速0.5 mL/min,检测波长210 nm,经外标法定量。【结果】L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8的线性范围均为5~100μg/mL,r值均为0.9999;检出限均为30 mg/kg,L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8加标回收率(n=6)范围为92.5%~105.9%,精密度(相对标准偏差,RSD)为0.5%~3.5%。【结论】该方法灵敏度高、专属性好、操作简便、重现性好,可用于化妆品中L-肌肽、乙酰基四肽-5、谷胱甘肽和乙酰基六肽-8的测定。[Objective]To establish a HPLC method for examination of L-carnosine,eyeseryl,glutathione and acetyl hexapeptide-8 in cosmetics.[Method]After being extracted by water,L-carnosine,Eyeseryl,Glutathione,and Acetyl Hexapeptide-8 were examined by HPLC with methanol-0.01%formic acid(V/V)aqueous solution as the mobile phase.The column was Agilent Eclipse XDB-C18(5μm,4.6 mm×150 mm).The flow rate was 0.5 mL/min.The determination wavelength was set at 210 nm.[Results]There was a good linear relationship within the range of 5-100μg/mL for L-carnosine,eyeseryl,glutathione,and acetyl hexapeptide-8.The recoveries of L-carnosine Eyeseryl Glutathione and Acetyl Hexapeptide-8 were between 92.5%~105.9%,with a RSD from 0.5%to 3.5%.[Conclusion]The method is simple,sensitive,specific and reproducible in the examination of L-carnosine,Eyeseryl,Glutathione,and Acetyl Hexapeptide-8 in cosmetics.

关 键 词：多肽 高效液相色谱法 L-肌肽 乙酰基四肽-5 谷胱甘肽 乙酰基六肽-8 化妆品 

分 类 号：R917[医药卫生—药物分析学]