青光眼滤过泡瘢痕化过程中转化生长因子-β的临床作用分析  

Effect of the transforming growth factor-βin the scarring process of glaucoma filtering bleb

作　　者：陆艳 王敏 曹西友 Lu Yan;Wang Min;Cao Xiyou(Department of Ophthalmology,Liqun Hospital of Putuo District of Shanghai,Shanghai 200333,China)

机构地区：[1]上海市普陀区利群医院眼科,200333

出　　处：《临床眼科杂志》2021年第4期357-360,共4页Journal of Clinical Ophthalmology

摘　　要：目的分析青光眼滤过泡瘢痕化过程中转化生长因子-β的临床作用。方法选取64只健康雄性SD大鼠,随机等分为A、B、C、D、E、F、G、H组,每组8只。A组为对照组,B-H组建立右眼球结膜滤过泡模型,术后观测滤过泡形成情况并监测眼压。B-G组分别于术后1、3、5、7、14、21 d及28 d处死,分析术区转化生长因子-β1(TGF-β1)及转化生长因子-β2(TGF-β2)的表达量。结果(1)B-H组均在术后成功建立球结膜滤过泡,滤过泡生存时间7~23 d,平均(13.26±5.73)d;(2)术眼在术后眼压先下降后回升至正常,术后1~5 d术眼眼压低于正常组,差异具有统计学意义(P<0.05);(3)Western印迹法显示TGF-β1及TGF-β2均在术后1 d明显增高,TGF-β1持续至术后5 d达峰值,TGF-β2持续至术后7 d达峰值,随后均逐渐降低至术后28 d,但表达量仍高于对照组,差异具有统计学意义(P<0.05)。结论TGF-β1及TGF-β2参与球结膜滤过泡瘢痕化过程,两者表达量的增加与瘢痕化进程一致。Objective To analyze the clinical effect of the transforming growth factor-β(TGF-β)in the scarring process of glaucoma filtering bleb.Methods TSixty four healthy male SD rats were randomly divided into eight groups as follows,group A,B,C,D,E,F,G and H with 8 rats in each group.Group A was the control group,and group B-H was used to establish the conjunctival bleb model in the right eye.The formation of filter bubble and intraocular pressure(IOP)were assessed after the operation.In group B-G,the rats were sacrificed at the 1st,3rd,5th,7th,14th,21th and 28th day after the operation and the expression levels of TGF-β1 and TGF-β2 in the operation area were analyzed.Results T(1)In group B-H,the filtering blebs were successfully established after the operation,and the survival time of filtering blebs ranged from 7 to 23 days,with an average of(13.26±5.73)days.(2)The IOP decreased at first and then returned to normal after the operation,and the IOP in group B,C and D were lower than that in the normal group,with statistical significant difference(P<0.05).(3)Western blot analysis showed that TGF-β1 and TGF-β2 increased significantly at the 1st day after the operation.TGF-β1 peaked at 5 days after the operation and TGF-β2 peaked at the 7th days after the operation,and then gradually decreased at the 28th day after the operation,but the expression level of TGF-β1 was still higher than that of the control group(P<0.05).Conclusions TTGF-β1 and TGF-β2 were involved in the scarring process of bulbar conjunctival filtering blebs,and the increase of their expression level were consistent with the scarring process.

关 键 词：青光眼滤过性手术 瘢痕化 转化生长因子-Β 

分 类 号：R73[医药卫生—肿瘤]