肾细胞癌外周血中长链非编码RNA差异表达分析  

Different expression of lncRNA in peripheral blood of renal cell carcinoma

作　　者：公海凤 褚微 张冰[2] 郑静[3] 苏庆国[2] 梅延辉[2] 纪洪[1] GONG Haifeng;CHU Wei;ZHANG Bing;ZHENG Jing;SU Qingguo;MEI Yanhui;JI Hong(Department of Pathology,Binzhou Medical University Hospital,Binzhou 256603,Shandong,P.R.China;Department of Urology,Binzhou Medical University Hospital,Binzhou 256603,Shandong,P.R.China;Clinical Medicine Laboratory,Binzhou Medical University Hospital,Binzhou 256603,Shandong,P.R.China)

机构地区：[1]滨州医学院附属医院病理科,山东滨州256603 [2]滨州医学院附属医院泌尿外科,山东滨州256603 [3]滨州医学院附属医院临床实验中心,山东滨州256603

出　　处：《滨州医学院学报》2021年第4期261-265,269,共6页Journal of Binzhou Medical University

基　　金：山东省医药卫生科技发展计划(2015WS0494,2018WS547);山东省自然科学基金(ZR2020MC068)。

摘　　要：目的探讨肾细胞癌(RCC)外周血中长链非编码RNA(lncRNA)及mRNA的表达情况。方法选择5例病理学诊断RCC患者的外周血血浆为实验组,同期5例健康人的外周血血浆作为对照组,采用基因芯片技术检测lncRNA及mRNA表达情况,实时荧光定量PCR进行验证分析。结果与对照组相比,实验组RCC患者外周血中3664个lncRNA异常表达(fold change≥2,P<0.05),其中1511个lncRNA上调表达,2153个lncRNA下调表达;mRNA差异表达有2268个(fold change≥2,P<0.05),其中932个mRNA上调表达,1336个mRNA下调表达。随机挑选上调表达的ENST00000547549、ENST00000591689及下调表达的NR_038263进行实时荧光定量PCR验证,其检测结果与芯片检测结果一致。结论lncRNA基因芯片是检测RCC外周血差异表达基因高效、快速的检测手段。外周血中异常表达的lncRNA与mRNA可能是RCC潜在诊断及随访的生物分子,并可能成为治疗的靶点。Objective To investigate the expression of long noncoding RNA(lncRNA)and mRNA in peripheral blood of renal cell cancer(RCC).Methods Five RCC patients diagnosed by pathology were selected as the case group,and the other five healthy persons who were routinely examined in the outpatient clinic were selected as the control group.All the cases were extracted the peripheral blood and the expression of lncRNA and mRNA were detected by gene chip technology.Two upregulated lncRNA ENST00000547549,ENST00000591689 and one down-regulated lncRNA NR_038263 were selected randomly for verification analysis by real-time fluorescence quantitative PCR.Results Compared with those in the control group,3664 lncRNA were differentially expressed in the peripheral blood,of which 1511 lncRNA were up-regulated,2153 lncRNA were down-regulated(fold change≥2,P<0.05).And 2268 mRNA were differentially expressed,of which 932 mRNA were up-regulated,and 1336 mRNA were down-regulated(fold change≥2,P<0.05).The verification analysis of the three lncRNA ENST00000547549,ENST00000591689 and NR_038263 by real-time quantitative PCR,and the results were consistent with the results of the chip detection.Conclusion The lncRNA gene chip is a high-efficiency and rapid detection method for detecting differentially expressed genes in peripheral blood of RCC.The differentially expressed lncRNA and mRNAs in peripheral blood may be potential biomolecules for the diagnosis and follow-up of RCC,and may become therapy targets.

关 键 词：肾细胞癌 长链非编码RNA 外周血 差异表达 

分 类 号：R36[医药卫生—病理学]