生物活性玻璃45S5体外对根尖牙乳头细胞成牙本质方向分化的影响  

Effect of bioactive glass 45S5 on odontogenic differentiation of apical papilla cells in vitro

作　　者：崔彩云 董艳梅[2] 邱莞迪 刘玉三[1] 王雯虹 李言君[1] CUI Caiyun;DONG Yanmei;QIU Wandi;LIU Yusan;WANG Wenhong;LI Yanjun(Department of Oral Medicine,Binzhou Medical University Hospital,Binzhou 256603,Shandong,P.R.China;Department of Cariology and Endodontology,Peking University School and Hospital of Stomatology&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology,Beijing 100081,P.R.China;Department of Pediatric Stomatology,Yantai Laishan Hospital of Stomatology,Yantai 264003,Shandong,P.R.China)

机构地区：[1]滨州医学院附属医院口腔内科,山东滨州256603 [2]北京大学口腔医学院·口腔医院牙体牙髓科,口腔数字化医疗技术和材料国家工程实验室,口腔数字医学北京市重点实验室,北京100081 [3]烟台莱山口腔医院儿童口腔科,山东烟台264003

出　　处：《滨州医学院学报》2021年第4期281-286,317,共7页Journal of Binzhou Medical University

基　　金：山东省医药卫生科技发展计划(2017WS552);山东省自然科学基金(ZR2019PH083)。

摘　　要：目的明确生物活性玻璃(45S5)对人根尖牙乳头细胞体外成牙本质方向分化的作用。方法浓度为0.1 mg/mL的45S5浸提培养液与人根尖牙乳头细胞共培养,离子体发射光谱检测45S5浸提培养液硅、钙、磷离子浓度,细胞活性噻唑蓝比色法检测细胞增殖,Realtime-PCR检测细胞成牙本质方向分化相关基因牙本质涎磷蛋白(DSPP)、牙本质基质蛋白(DMP-1)的表达,茜素红矿化结节染色及氯化十六烷基吡啶钙结节半定量分析矿化结节形成。结果与对照组相比,45S5浸提培养液中硅离子浓度显著升高(P<0.05);45S5浸提培养液连续诱导培养根尖牙乳头细胞3、5、7 d后,与对照组相比显著促进细胞增殖(P<0.05);45S5浸提培养液连续诱导培养根尖牙乳头细胞7 d,细胞DSPP、DMP-1的表达量显著高于对照组(P<0.05);45S5生物活性玻璃体浸提培养液连续诱导培养根尖牙乳头细胞14和21 d,茜素红染色后观察45S5组和矿化诱导培养液(OM)组均见明显红染矿化结节,对照组未见明显红染矿化结节;氯化十六烷基吡啶钙结节半定量分析各组钙离子浓,45S5和OM组OD值高于对照组(P<0.05),45S5组OD值高于OM组(P<0.05)。结论0.1 mg/mL生物活性玻璃45S5体外促进根尖牙乳头细胞增殖、成牙本质方向分化相关基因高表达和矿化结节形成,45S5体外促进根尖牙乳头细胞成牙本质方向分化。Objective To determine the effect of bioactive glass 45S5 on odontoblast differentiation of human apical dental papilla cells in vitro.Methods The human apical papilla cells were co-cultured in the extracted culture medium with 0.1mg/mL 45S5.The inductively coupled plasma analysis was used to test the concentrations of silicon,calcium and phosphorus in the 45S5 extract.Cell proliferation was detected by thiazolyl blue colorimetry.Realtime-PCR was used to detect the expression of DSPP and DMP-1 genes related to odontogenic differentiation of apical papilla cells.With alizarin red mineralized nodule staining,acetylpyridine chloride calcium nodule was used to semi-quantitatively analyze the mineralized nodule formation of apical papilla cells.Results Compared with that in the control group,the concentration of silicon ion in 45S5 extraction medium increased significantly in the control group(P<0.05).The proliferation of apical dental papilla cells induced by 45S5 extract on the 3rd,5th and 7th day was significantly higher than that in the control group(P<0.05).The expression of DSPP and DMP-1 in apical papilla cells induced by 45S5 extract for 7 days was higher than that in control group.Calcium acetylpyridine chloride nodules were semi-quantitatively analyzed for the formation of mineralized nodule of apical papilla cells in each group.OD values of silicon and OM groups were higher than those in the control group(P<0.05),the OD value of silicon group was higher than that of OM group(P<0.05).Conclusion Bioactive glass 45S5 in concentration of 0.1 mg/mL promotes the proliferation of apical papilla cells,the high expression of genes related to odontogenic differentiation and the formation of mineralized nodules in vitro.45S5 promotes dentin differentiation of apical papilla cells in vitro.

关 键 词：生物活性玻璃 根尖牙乳头细胞 成牙本质分化 

分 类 号：R780.2[医药卫生—口腔医学]