miR-203-3p在肝癌细胞中的表达及其对细胞增殖、迁移和侵袭的影响  

The expression of miR-203-3p in hepatocellular carcinoma cells and the effects of cell proliferation,migration and invasion

作　　者：陈昶舟 赵海潮 付西峰 赵浩亮[1,2] CHEN Changzhou;ZHAO Haichao;FU Xifeng;ZHAO Haoliang(Shanxi Medical University,Shanxi Taiyuan 030000,China;Department of General Surgery,Shanxi Bethune Hospital,Shanxi Taiyuan 030032,China)

机构地区：[1]山西医科大学,山西太原030000 [2]山西白求恩医院普通外科,山西太原030032

出　　处：《现代肿瘤医学》2021年第18期3133-3138,共6页Journal of Modern Oncology

基　　金：山西省科技厅项目(编号:201901D111404);山西省教育厅项目(编号:2020SY283)。

摘　　要：目的:探讨微小RNA-203-3p(miR-203-3p)在肝细胞癌细胞中的表达,并进一步分析其对肝细胞癌进展的影响。方法:通过qRT-PCR检测miR-203-3p在不同肝细胞癌细胞系中的表达,并利用敲低和过表达的方法分析miR-203-3p表达水平的变化对肝癌细胞生长、运动等的影响,并初步探索其机制。结果:肝细胞癌细胞中的miR-203-3p表达水平低于正常肝细胞。功能上,miR-203-3p抑制肝细胞癌细胞增殖、迁移、侵袭,加速细胞凋亡;机制上,miR-203-3p可与FGF2 mRNA碱基配对,吸附FGF2 mRNA导致FGF2蛋白生成减少。结论:miR-203-3p通过直接靶向作用,抑制FGF2表达,从而抑制肝细胞癌细胞的增殖、迁移和侵袭。Objective:To investigate the expression of miR-203-3p and its effect on hepatocellular carcinoma.Methods:We used qRT-PCR to detect the expression of miR-203-3p in different hepatocellular carcinoma(HCC)cell lines,and knock-down and over-expression were carried out in HCC cell lines respectively to test cell proliferation,migration,invasion.Results:Compared with normal liver cells,the expression level of miR-203-3p in hepatocellular carcinoma cells was significantly reduced.Functionally,miR-203-3p can inhibit hepatocellular carcinoma cell proliferation,migration,invasion,and accelerate cell apoptosis.At the same time,miR-203-3p reduced the migration and invasion.From the mechanism point of view,miR-203-3p can base pair with FGF2 mRNA,leading to the knockdown of FGF2 mRNA and the downregulation of FGF2 protein.Conclusion:miR-203 can inhibit the proliferation,invasion and metastasis of hepatocellular carcinoma cells by inhibiting the expression of FGF2.

关 键 词：微小RNA-203-3p 成纤维细胞生长因子2 肝细胞癌 肿瘤进展 

分 类 号：R735.7[医药卫生—肿瘤]