人结直肠癌组织Claudin-1、Claudin-7蛋白的表达与患者预后的关系  

Association of Claudin-1 and Claudin-7 expression in human colorectal cancer with postoperative prognosis

作　　者：曹跃鹏[1] 袁旦平[1] 庞洪双[1] 赵振亚[1] 李璐 陶勇 CAO Yuepeng;YUAN Danping;PANG Hongshuang;ZHAO Zhenya;LI Lu;TAO Yong(Ningbo No.1 Hospital,Ningbo 315010,Zhejiang,China)

机构地区：[1]宁波市第一医院,宁波315010 [2]宁波市临床病理诊断中心

出　　处：《现代实用医学》2021年第7期851-854,861,F0002,共6页Modern Practical Medicine

基　　金：宁波市自然科学基金项目(2019A610335);浙江省医药卫生科技计划项目(2020KY248);宁波市公益类科技计划项目(202002N3163)。

摘　　要：目的探讨人结直肠癌组织Claudin-1、Claudin-7蛋白的表达与患者预后的关系。方法收集88例结直肠癌患者的术后标本及临床资料。运用免疫组织化学方法检测结直肠癌组织与癌旁组织的Claudin-1、Claudin-7蛋白表达水平,分析其表达与结直肠癌患者临床病理特征及预后的关系。结果免疫组化结果显示,Claudin-1、Claudin-7蛋白阳性黄褐色颗粒主要分布在细胞膜或细胞质中。Claudin-1蛋白在癌旁组织中呈低表达22.72%(20/88),而在结直肠癌组织中呈高表率达80.68%(71/88);Claudin-7蛋白在癌旁组织中呈高表达90.91%(80/88),而在结直肠癌组织中低表达率达45.45%(40/88);差异均有统计学意义(均P<0.05)。Claudin-1及Claudin-7蛋白表达与结直肠癌患者的浸润深度、分化程度、淋巴转移、TNM分期均有关(均P<0.05)。随访显示Claudin-1蛋白阳性表达组与Claudin-1蛋白阴性表达组5年总生存率分别为27.91%和49.86%,Claudin-7蛋白阳性表达组与Claudin-7蛋白阴性表达组5年总生存率分别为48.70%和27.90%;两组差异均具有统计学意义(均P<0.05)。Cox多因素分析显示,TNM分期晚、浸润程度深、淋巴结转移和Claudin-1蛋白阳性表达及Claudin-7蛋白阴性表达均是结直肠癌患者术后总生存期的独立高危因素(均P<0.05)。结论Claudin-1高表达及Claudin-7低表达可以促进结直肠癌的发生发展,与结直肠癌的侵袭和转移有关,并会影响患者预后,对判断手术后结直肠癌患者预后具有较好的参考价值。Objective To investigate the association of Claudin-1 and Claudin-7 protein expression in colorectal cancer with its prognosis.Methods Clinical data and resection samples of 88 colorectal cancer patients identified by pathology undergoing resection were collected.Claudin-1 and Claudin-7 expressions in colorectal cancer tissues and matched adjacent noncancerous tissue collected were detected by immunohistochemical staining.The associations of Claudin-1 and Claudin-7 protein expression level with overall survival,clinicopathologic features were analyzed by Chi-square test,Cox regression analysis,Kaplan-Meier analysis and rank test.Results Immunohistochemical staining showed that Claudin-1 and Claudin-7 protein-positive yellow-brown particles were mainly distributed in the cell membrane or cytoplasm,and Claudin-1 protein was highly expressed in colorectal cancer tissues(80.68%,71/88),while lowly expressed in adjacent tissues(22.72%,20/88).Besides,Claudin-7 protein was highly expressed in adjacent tissues(90.91%,80/88),while lowly expressed in colorectal cancer tissues(45.45%,40/88).They were all statistically significant(allP<0.05).Combined with clinicopathological features,the expression of Claudin-1 and Claudin-7 protein was correlated with the depth of invasion,differentiation,lymphatic metastasis and TNM stage of colorectal cancer patients(allP<0.05).The follow-up results showed that patients with Claudin-1 protein positive and Claudin-7 protein negative expression had worse survival than other groups(allP<0.05).Cox regression analysis showed that poorer TNM stage(HR=3.82,95%2.02~6.31,P<0.05),the deeper of invasion(HR=1.32,95%1.21~3.22,P<0.05),lymph node positive metastasis(HR=1.95,95%1.31~3.96,P<0.05),Claudin-1 protein positive expression(HR=3.29,95%CI1.66~5.64,P<0.05)and Claudin-7 protein negative expression(HR=2.07,95%CI1.23~4.31)were independent risk factors for the overall survival of patients with colorectal cancer(P<0.05).Conclusions High expression of Claudin-1 and low expression of Claudin-7 can promote

关 键 词：结直肠肿瘤 癌 CLAUDIN-1 Claudin-7 预后 

分 类 号：R735[医药卫生—肿瘤]