番茄节间长度基因IL10和IL11的鉴定和功能验证  

Identification and Functional Verification of Tomato Stem Internode Length Genes IL10 and IL11

作　　者：刘根忠 石春美 于会洋 王莹 陈卫芳 尚乐乐 张余洋[1] 叶志彪[1] LIU Genzhong;SHI Chunmei;YU Huiyang;WANG Ying;CHEN Weifang;SHANG Lele;ZHANG Yuyang;YE Zhibiao(Key Laboratory of Horticultural Plant Biology,Ministry of Education,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]华中农业大学园艺植物生物学教育部重点实验室,武汉430070

出　　处：《园艺学报》2021年第7期1340-1348,共9页Acta Horticulturae Sinica

基　　金：国家重点研发计划项目(2016YFD0100204-21,2018YFD1000800);国家自然科学基金项目(31991182,31972426);武汉市应用基础前沿项目(2019020701011492)。

摘　　要：通过对266份番茄核心种质资源第3穗果实处节间长度进行测定,进行全基因组关联分析,共鉴定7个与节间长度相关的QTL位点。对10和11号染色体中的显著性SNP进行候选基因分析,进一步挖掘控制番茄节间长度的主效基因IL10(Forkhead-associated domain protein)和IL11(Auxin-regulated protein)。以‘Ailsa Craig’番茄为背景材料,通过农杆菌介导的方法进行遗传转化,获得了11株IL10基因沉默、10株IL11基因沉默的株系,其节间长度比野生型短。综上所述,IL10和IL11为控制番茄节间长度的关键基因,对番茄节间长度起正向调控作用。We measured and analyzed internode length at the third panicle of 266 tomato accessions.Seven QTL related to tomato internode length were identified by genome-wide association study.Candidate genes were analyzed based on the significant SNPs in chromosomes 10 and 11.The IL10(Forkhead-associated domain protein)and IL11(Auxin-regulated protein)related to the internode length were further excavated.Tomato‘Ailsa Craig’was used as the background material.11 IL10-RNAi lines and 10 IL11-RNAi lines were obtained by Agrobacterium-mediated transformation.The internode length of IL10-and IL11-silencing transgenic lines was significantly shorter than that of the wild type.To sum up,IL10 and IL11 are the key genes that positively regulates tomato internode length.

关 键 词：番茄 茎秆 节间长度 全基因组关联分析 候选基因 

分 类 号：S641.2[农业科学—蔬菜学]